瑞士乳杆菌发酵乳中多肽的分离对小鼠体外淋巴细胞增殖的影响

Effects of milk fermented by Lactobacillus helveticus on lymphocyte proliferation in vitro

目的:分离瑞士乳杆菌发酵乳中多肽片段,并对分离片段的的免疫调节作用进行研究。方法:利用瑞士乳杆菌LH0906发酵牛乳获得多肽样品,采用离心、超滤的方法对所得多肽进行粗提,利用凝胶层析色谱和反相层析色谱进一步分离发酵乳中的多肽,使用Folin-Lowry法测定发酵后样品中多肽含量,采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)比色法检测发酵乳中的多肽对小鼠脾脏淋巴细胞体外增殖的影响。结果:牛乳发酵液其对小鼠脾脏淋巴细胞增殖效果显著高于未发酵牛乳,并经过凝胶层析色谱和反相层析色谱分离发酵乳得到的峰S12-A对小鼠脾脏淋巴细胞体外增殖效果十分显著(P<0.01),峰S12-C对小鼠脾脏淋巴细胞体外增殖效果显著(P<0.05)。结论:牛乳经瑞士乳杆菌LH0906发酵后,分离得到的多肽片段对小鼠脾脏淋巴细胞体外增殖具有显著的增强作用。

Objective： To isolate the polypeptide in fermented milk by Lactobacillus helveticus and study its immunomodulating effect.Methods： Milk was fermented by Lactobacillus helveticus.The samples were separated to prepared peptides by size-exclusion chromatography and reversed-phase chromatography after centrifuging and ultrafiltration.Folin-Lowry method was used to measure the content of the peptide.Then the effects of isolated peptide fractions on the murine splenocytes proliferation in vitro were evaluated by 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） assay.Results： The bioactive peptides released during milk fermentation by Lactobacillus helveticus has a significant effect（P0.01） on lymphocyte proliferation in vitro.