胭脂虫中洋红酸的C_(18)-HPLC-PDA检测

C_(18)-HPLC-PDA assessment of carminic acid from Dactylopius coccus Costa

目的:建立一种对胭脂虫中的洋红酸进行定性、定量分析的C18-HPLC方法,对我国四川西昌地区产胭脂虫中的洋红酸含量进行初步评估。方法:(1)用pH=9的0.0125 mol/L的Na2CO3溶液萃取洋红酸;(2)最终HPLC分离条件:固定相:SUPERIDREX ODS C18色谱柱(39 mm×15 cm,4μm);流动相A:甲醇-水(2:8v,/v),用磷酸调pH为5;流动相B:甲醇;线性梯度洗脱:B在15 min内由0增至100%;流速:0.8 mL/min;PDA波长收集范围为250～630 nm;色谱图检测波长:533 nm;进样量:20 mL。(3)用外标法测定样品中洋红酸的含量。结果:我国西昌地区产胭脂虫中洋红酸的含量为307.02 mg/g。

Objective： This study aims to develop a C18-HPLC method for the identification and quantification of carminic acid and then give a preliminary assessment of carminic acid amount from Dactylopius coccus Costa cultured in Xichang,Sichuan province,China.Methods：（1）Carminic acid was extracted by 0.0125 mol/L Na2CO3（pH=9） solution;（2）Final HPLC conditions： Stationary phase： SUPERIDREX ODS C18 column（39 mm x 15 cm,4 μm）;Mobile phase A： methanol-water（2：8,v/v） with pH of 5 by phosphoric acid;Mobile phase B： methanol;Linear gradient： variation in B rate from 0 to 100% in 15 minutes;Flow rate： 0.8 mL/min;250～630 nm Spectra acquisition by PDA;Chromatographic monitoring wavelength： 533 nm;Sample injection volume： 20 mL;（3）Amount assessment of carminic acid by external sample.Results： The amount of carminic acid from Dactylopius coccus Costa cultured in Xichang,Sichuan province,China is 307.02 mg/g.