应用全胚胎培养方法研究野百合碱对小鼠胚胎发育的影响

Toxicity of monocrotaline on in vitro cultured mouse embryos

目的:探讨野百合碱的胚胎发育毒性。方法:采用小鼠全胚胎培养模型,将8.5 d小鼠胚胎在含有野百合碱的即刻离心血清中培养48 h(野百合碱的终浓度分别为100,50,25,12.5 mg.L-1),观察野百合碱对胚胎生长发育(卵黄囊直径、颅臀长、头长、体节数)和组织器官形态分化(卵黄囊循环、尿囊、翻转、心、脑、尾神经管、视听嗅系统、腮弓、颌突、肢芽)的影响。结果:随野百合碱浓度的增加,胚胎的生长发育和组织器官形态分化受到的影响越来越严重,其中以视听系统、颌突、肢芽等项的影响最为明显。结论:野百合碱对体外培养的小鼠胚胎有明显的毒性作用,提示妊娠期暴露于该化合物对胎儿具有潜在的毒性。

Objective ： To investigate the fetotoxicity of monocrotaline. Method ： Mouse whole embryo culture （WEC） was applied. Post-implantation （8.5 d） mouse embryos were isolated from their mothers and put into the medium of immediately centrifuged serum （ICS） prepared from rats. Different concentrations of monocrotaline （ 100,50,25,12. 5 mg·L-1 ） were added into the WEC. Development （yolk sac diameter, crown-rump length, head length, somite number）and organic morphodifferentiation （yolk sac circulation, allantois, embryonic flexion, heart, brain, optic-otic-olfactory organ, branchial arch, maxillary, mandible, bud） of embryos were observed at 48 h after treatment. Result： Obvious fetotoxicity could be observed in various monocrotaline treatment groups in a dose-dependent manner. Development of embryos was delayed slgnificantly at dose 12. 5-100 mg L-l. Malformations were shown in all organic morphodifferentiation indice, especially in opti-otic organ, mandible and bud. Conclusion： Monocrotaline had obvious fetotoxicity in vitro WEC, indicating that exposure of pregnant mice to monocrotaline may have potential risk on fetus.