可疑翼手参皂苷colochiroside A的抗肿瘤活性研究

Studies on antitumor activities of triterpene glycoside colochiroside A from sea cucumber Colochirus anceps

目的:研究可疑翼手参体内三萜皂苷colochiroside A(CA)的体内外抗肿瘤活性。方法:采用MTT法检测三萜皂苷CA对6种体外培养肿瘤细胞(p388,HL-60,A-549,SpC-A4,MKN-28,SGC-7901)的增殖抑制活性;以小鼠肉瘤S180和小鼠肝癌H22为移植性肿瘤模型,观察CA的体内抗肿瘤活性及对免疫器官的影响。结果:CA对6种体外培养肿瘤细胞均显示显著抑制增殖作用,IC50平均值为(3.61±0.55)mg.L-1;对小鼠S180肉瘤的抑制率分别为36.4%,70.0%,对小鼠H22肝癌的抑制率分别为34.8%,43.9%,52.2%,对荷瘤小鼠的免疫器官未见明显影响。结论:可疑翼手参体内皂苷CA具有很强的体内外抗肿瘤活性,且不降低荷瘤宿主的器官指数,有希望研发为种新型的抗肿瘤化合物。

Objective： To study the antitumor activities of the triterpene glycoside colochiroside A （CA） from the sea cucumber Colochirus anceps. Method： The tests of antitumor activities in vitro and in vivo were applied to demonstrate the effect of CA. Result： The preliminary cytotoxic assay of CA exhibited significant eytotoxic activity against 6 types cultured tumor cell lines of p388, HL- 60, A-549, SpC-A4, MKN-28, and SGC-7901, the mean of IC50 were （3.61 ± 0. 55） mg L-1. The preliminary antitumor assay of CA indicated that this saponin exhibited high inhibiting activity against the H22 live cancer and the S180 sarcoma cells in mouse. The inhibition ratio to H22 liver cancer were 34. 8% , 43.9% and 52. 2% , while the ratio to S180 sarcoma were 36.4% , 70. 0%, the immunoregnlatory founction study indicated CA has not significant effect on the developments of thymus and spleen. Conclusion： The saponin CA exhibited remarkable antineoplastic activities in vitro and in vivo, and could not reduce the immunoregulatory founction of mice.