2种黔产淫羊藿总黄酮对免疫功能影响的比较研究

Immunologic functions of total flavone of Epimedium of two species in Guizhou

目的:比较2种黔产淫羊藿总黄酮(TFE)对CTX小鼠免疫功能的影响。方法:采用CTX造免疫低下小鼠模型,同时给药10 d,以体重、脾重、脾指数,血清溶血素浓度,血清TNF-α及IL-2含量为指标,观察2种TFE增强免疫作用,并比较其差异。结果:单次ip CTX 80 mg.kg-1,使正常小鼠的脾指数、血清溶血素水平、IL-2和TNF-α含量明显下降;ig 2种TFE1.3,0.65 g.kg-1 10 d,可使体重、脾重、睥指数上升(P<0.01),下降的溶血素值回升(P<0.01),以及下降的IL-2和TNF-α含量显著升高(P<0.01)。结论:2种黔产淫羊藿TFE对CTX小鼠的非特异免疫和特异免疫功能都具有明显增强作用;但2种黔产淫羊藿茎叶TFE增强免疫作用差异不明显。提示TFE中除了淫羊藿苷(ICA)外,尚有其他增强免疫的有效成分。

Objective： Comparative study on the immunologic functions of total flavones of Epimedium （TFE） in two species in Guizhou on CTX mice. Method ： To set UP a hypoimmunity mouse model by CTX and give TFE in 10 days, the enhancement of immunologic functions of TFE of two species were observed with the indice of the weight of body and spleen, the spleen-index, the serum level of hemolysin, TNF-α and IL-2. Result： The spleen-index, the level of hemolysin, TNF-α and IL-2 of the mice decreased apparently（P 〈0.01 ）by single ip in CTX 80 mg kg-1. The weight of body and spleen, the spleen-index of the mice increased, the decreased serum level of hemolysin TNF-α and IL-2 increased significantly （ P 〈 0.01 ）, by TFE of two species of 1.3, 0.65 g kg-1 from the mice ig. Conclusion ： The TFE of two species of Epimedium in Guizhou have an obvious immune enhancement effect on the non-specific immunity and specific immunity of CTX model mice. But the immune enhancement effects of the TFE from the stem and leaves of two species of Epimedium contrast insignificantly. This indicates that in addition to ICA, there must be other bioactive components that can enhance immunity.