红外二阶导数指纹图谱用于紫花地丁药材的产地分类

Application of Second Derivative IR Fingerprint Spectra to Origin Prediction of the TCM of Viola Yedoensis Makino

建立了紫花地丁药材的红外二阶导数指纹图谱。采用傅里叶变换-红外光谱法测定10个不同产地紫花地丁试样的红外光谱,红外分辨率为8 cm^-1,扫描范围为4 000-400 cm^-1,利用二阶导数重构指纹图谱后,进行系统聚类分析和Fisher′s判别分析。结果表明所建立的方法能够能有效地对样品产地进行预测,可为紫花地丁药材质量控制提供一种简便、有效的手段。

The second derivative of IR fingerprint spectra of the TCM,viola yedoensis Makino,were established.Samples of viola yedoensis Makino from 10 different origins were determined by FT-IR with resolution of 8 cm^-1 and scanning range of 4 000-400 cm^-1.The IR spectra obtained were transformed into its second derivative fingerprint spectra,and were analyzed by systematic clustering analysis and Fisher′s discriminant analysis.It was found that the proposed method can be used to predict effectively the origin of viola yedoensis Makino,and to provide a simple and rapid method for quality control of the TCM.