取食转Bt基因水稻褐飞虱对杀虫剂的敏感性及其代谢酶的活性

Insecticide Susceptibility and Activity of Metabolic Enzymes of Brown Planthopper,Nilaparvata lugens (Stl)(Hemiptera:Delphacidae) Feeding on Transgenic Bt Rice

测定比较了褐飞虱Nilaparvata lugens在转cry1Ab基因水稻KMD1和KMD2及其对照秀水11上取食不同世代的室内种群和田间种群对5种杀虫剂的敏感水平及其体内解毒酶(酯酶和谷胱甘肽S-转移酶)、靶标酶(乙酰胆碱酯酶)和保护酶(超氧化物歧化酶、过氧化氢酶和过氧化物酶)的活性,以及体内Cry1Ab蛋白的含量。结果表明,5种杀虫剂对分别持续取食KMD1和KMD2的褐飞虱室内种群和田间种群LD50值与取食对照的无显著差异。在KMD1和KMD2上取食1代和9代的褐飞虱室内种群以及田间种群体内解毒酶、靶标酶和保护酶等活性与取食对照的差异不显著。取食KMD的褐飞虱不同种群体内的Cry1Ab蛋白含量相对稳定,其中以取食KMD2的较高。上述结果表明,转cry1Ab基因水稻KMD1和KMD2对褐飞虱的杀虫剂敏感性和代谢酶活性无显著影响。

The toxicities of five commonly-used insecticides against different populations of rice brown planthopper,Nilaparvata lugens,fed on transgenic rice with cry1Ab gene,KMD1 and KMD2 for different generations were measured and compared with those on non-transgenic parental rice variety,Xiushui 11.Activities of detoxification enzymes（EST and GST）,target enzyme（AChE）,and protective enzymes（SOD,CAT and POD） from different populations of N.lugens fed on KMD1,KMD2,and Xiushui 11 and the content of Cry protein were also detected.For all five insecticides tested,there were no significant differences in LD50 values among different populations of N.lugens fed on KMD1 KMD2,and Xiushui 11 continuously in the laboratory and collected from the rice fields.For all six metabolic enzymes tested,there was no significant difference of enzyme activities among different populations of N.lugens fed on KMD1,KMD2 and Xiushui 11 for 1 and 9 generations in the laboratory or collected from the rice fields.The content of Cry protein was relatively stable in different populations of N.lugens fed on Bt rice,and was higher in N.lugens fed on KMD2.It was concluded that transgenic rice lines with cry gene,KMD1 and KMD2,had no significant effects on the susceptibility to insecticides and activities of metabolic enzymes of N.lugens.