青蒿琥酯对人宫颈癌HeLa和Siha细胞辐照后DNA损伤水平的影响

Study on the effect of artesunate combined with irradiation on DNA damage of HeLa and Siha cells of human cervical cancer

为研究青蒿琥酯联合照射对人宫颈癌HeLa和Siha细胞DNA损伤的影响,取对数生长期细胞,通过MTT比色法测定不同浓度青蒿琥酯作用24 h对HeLa和Siha细胞生长的抑制效应,将细胞分为单纯照射组和联合治疗组,每组分别给予0、2、4、6 Gy四个剂量的60Coγ射线照射,应用单细胞凝胶电泳(SCGE)法检测青蒿琥酯对HeLa和Siha细胞照射后DNA损伤的影响。MTT结果表明,青蒿琥酯对宫颈癌HeLa和Siha细胞的生长抑制呈剂量依赖性。单细胞凝胶电泳法检测发现:联合治疗组HeLa细胞经0、2、4和6 Gy 60Coγ射线辐照后彗星细胞的尾部DNA百分数及Olive尾矩明显大于单纯照射组,差别有统计学意义(P<0.05),而Siha细胞联合治疗组与单纯辐照组相比,无明显差异。实验结果表明,青蒿琥酯对p53突变型子宫颈癌HeLa细胞具有明显的放射增敏作用,而对p53野生型子宫颈癌Siha细胞没有明显的放射增敏作用。

In order to investigate the effect of artesunate combined with irradiaton on DNA damage of HeLa and Siha cells of human cervical cancer, HeLa and Siha cells were cultured in vitro and exposed to different concentration of artesunate for 24 h and MTT assay was used to observe the inhibitory effect of different concentrations of artesunate on the proliferation of HeLa and Siha cells. The cells were divided into 2 groups as the irradiated group and the union treatment group. Here it was set up four absorbed doses of 60↑Coγ-irradiation in each group with 0, 2, 4, and 6Gy, and the DNA damage were detected by single cell gel electrophoresis assay. MTT analysis showed that the inhi- bition of artesunate on HeLa and Siha cells of cervical cancer was in concentration-dependent manners. Single cell gel eleetrophoresis showed that the DNA damage of HeLa cells treated with artesunate was more serious than that treated only with irradiation （P〈0.05）, but had no such effect on Siha cells. Artesunate can increase the radio-sensitivity of HeLa cells of cervival cancer with p53 mutant, but has no such effect on wide type p53 Siha ceils.