高脂喂养联合链脲佐菌素诱导大鼠2型糖尿病模型研究

Type 2 rat diabetic models induced by high-fat feeding combined with Streptozocin

目的：进行高脂喂养联合链脲佐菌素（STZ）诱导大鼠2型糖尿病模型的研究。方法：5—6周SD大鼠21只分普通与高脂饲料喂养4周后按体重腹腔注射（ip）柠檬酸钠缓冲液或STZ溶液；测定注射STZ1周后的空腹血糖（FBG）、血脂（三酰甘油TG、总胆囿醇T—CHO）、血清胰岛素（INs）；将FBG≥7．0mmol／L的大鼠定为糖尿病大鼠。成模后均连续观察4周，观察内容包括：体重、水量、尿量、糖耐量（OGTT）测试、TG、T—CHO、INS。5—6周SD大鼠58只运用高脂喂养联合STZ（45mg／kg）诱导大鼠2型糖尿病方法制备模型，连续给予糖尿病大鼠治疗2型糖尿病药物盐酸二甲双胍（200mg／kg）、马来酸罗格列酮（3、6、12mg／kgL20）、格列美脲（2_3]、4、8mg／kg）5d，研究该模型的有效性。结果：注射STZ1周后B组（高脂喂养＋30mg／kgSTZ）没出现糖尿病“三多一少”典型症状，FBG值未达成模标准；C组（高脂喂养＋45mg／kgSTZ）出现糖尿病典型症状、FBG值显著升高且达成模标准；盐酸二甲双胍（200mg／kg）、格列美脲（2mg／kg）对此方法诱导出来的糖尿病大鼠有显著的降糖作用。结论：高脂喂养联合STZ（45mg／kg）诱导大鼠2型糖尿病的方法有效且模型长期稳定。

Objective To study type 2 rat diabetic models induced by high - fat feeding combined with STZ. Method Five to six weeks old SD rats were injected with low doses of STZ after being fed with high - fat and high - sugar diets for 4 weeks. One week thereafter their blood fasting glucose （ FBG）, triglyceride （ TG）, total cholesterol （ T - CHO）, and serum insulin （ INS ） levels were measured ; rats with FBG higher than 7.0 mmol/L were classified as diabetic models. The models were then subjected to continuous monitoring of body weight ,water consumption and urine collection, OGTT, TG, T - CHO, and INS. These models effectiveness was checked by continuous doses of Mefformin Hydrochloride （ 200 mg/kg） , Rosiglitazone Maleate （ 3,6,12 mg/kg）, Glimepiride （2,4,8 mg/kg） for 5 d, which were typical treatments for type 2 diabets. Results Group B （ Low dose group,with high fat＆sugar diets and 30 mg/kg STZ） did not develop diabetic symptoms and FBG was lower than 7.0 mmol/L;while group C （ High dose group ,with high fat＆sugar diets and 45 mg/kg SZT） developed diabetic symptoms,its FBG was above the required level, while Mefformin Hydroehloride（200 mg/kg）, Glimepiride（2 mg/kg）could indeed significantly bring their sugar levels down. Conclusion The method of inducing type 2 rat diabetes by high - fat feeding combined with STZ is effective and the models are stable for a long period of time.