外源性PDGF-DD蛋白对肝癌细胞株BEL-7402的体外作用

The effect of exogenous PDGF-DD protein on human hepatic carcinoma cell line BEL-7402 in vitro

目的:探讨外源性PDGF-DD蛋白对PDGF-D和β-PDGFR阳性表达的人肝癌细胞株BEL-7402增殖和转移相关基因VEGF及CXCR4表达的影响。方法:浓度为0～100pg/mL PDGF-DD蛋白作用于肝癌细胞株BEL-740248h,MTT检测细胞活性;流式细胞仪检测细胞周期;RT-PCR检测VEGFmRNA和CXCR4 mRNA表达。结果:外源性PDGF-DD蛋白干预细胞后,可促进细胞增殖,且在0～100pg/mL范围内呈剂量依赖性;周期分布显示G0/G1期细胞比例下降,S期细胞比例增多;且上调VEGFmRNA和CXCR4 mRNA的表达。结论:外源性PDGF-DD能促进BEL-7402细胞增殖,上调VEGF、CXCR4 mRNA的表达。提示PDGF-D及其信号传导系统在肝癌的发生、发展及转移中可能发挥重要的作用,可作为肝癌预后预测指标和治疗靶点。

Objective To investigate the effect of exogenous PDGF-DD protein on proliferation and metastasis related gene （VEGF and CXCR4） of PDGF-D and β-PDGFR positive expressed human hepatic carcinoma cell line BEL-7402. Methods BEL-7402 cells were exposed to different concentrations of PDGF-DD protein（0,5, 10,20,50,100 μg/mL） for 48 h, and cell proliferation was detected by MTT assay. The flow cytometer （FCM） was used to analyze the distribution of cells cycle. The mRNA expressions of VEGF and CXCR4 were detected by RT- PCR. Results Exogenous PDGF-DD protein promoted proliferation of cells in a dose-dependent manner from 10 pg/mL to 100 pg/mL （P 〈 0.05）. Incubation of tumor cells with PDGF-DD markedly decreased the percentage of the G0/G1 -phase and increased that of the S-phase. Compared with control group （0 pg/mL）, mRNA expression of VEGF and CXCR4 （from 10 pg/mL to 100 pg/mL） were increased （P 〈 0.05）. Conclusions Exogenous PDGF-D signaling may play a crucial role in the carcinogenesis, development and metastasis of hepatocellular carcinoma. It can be used as a prognostic factor and a potential target for therapy in hepatocellular carcinoma.