摘要
目的探讨川芎嗪对大鼠心肌缺血再灌注损伤时细胞凋亡的影响及可能的机制。方法雄性SD大鼠36只随机分三组:假手术组、模型组、川芎嗪治疗组,每组12只。用结扎大鼠左冠状动脉前降支法制备心肌缺血再灌注模型,测定再灌注前静脉注入川芎嗪对损伤大鼠心肌组织中MDA,SOD生成的影响;免疫组化法检测大鼠心肌细胞Bcl-2、Bax基因表达。结果①川芎嗪组能减少心肌组织中MDA生成,并明显提高缺血再灌注组织中SOD的活力;②川芎嗪组中bcl-2表达明显增多,平均灰度值明显下降,与模型组比较有显著差异(P>0.01),bax表达下降,但无统计学意义(P>0.05)。结论川芎嗪可能通过拮抗氧自由基、上调凋亡抑制基因bcl-2等作用,对心肌缺血再灌注损伤时细胞凋亡有一定的防治作用。
Objective The objective of the present study is to investigate the Effects and mechanism of chuan xiong qin on cardiomyocyte apoptosis after ischemia reperfusion.Methods 36 male Sprague- Dauley rats were randomly divided into Three groups：sham operating group, ischemia reperfusion group, treatment group,which group had 12 rats. The left anterior descended（LAD）coronary artery was ligated to establish the ischemia/reperfusion heart model. The rats were injected chuan xiong qin before reperfusion. The concentrations of SOD,MDA in myocardial were measured; The expression of Bax and Bcl-2 genes were investigated by immunohistochemistry.Results ①chuan xiong qin decreased the MDA contents, improved the SOD activities;②chuan xiong qin donot inhibit the expression of bax gene but significantly increased expression of Bcl-2 gene.Conclusion The results indicate that chuan xiong qin can inhibit apoptosis induced by ischemia-reperfasion injury through resisting the damaging of free adical and increasing the expression of Bcl-2 gene.
出处
《中国实用医药》
2011年第2期29-30,共2页
China Practical Medicine
