IQGAP1在肺癌中的表达及其临床意义

Expression and clinical significance of IQGAP1 in lung cancer

目的探讨肺癌中支架蛋白IQGAP1的蛋白表达水平,及IQGAP1对肺癌细胞运动侵袭能力的影响。方法 RT-PCR检测IQGAP1mRNA的表达,免疫组织化学SABC法及Western blot检测IQ-GAP1蛋白的表达,并采用Transwell实验探讨IQGAP1蛋白促进侵袭转移的作用机制。结果研究发现其在肺癌组织存在高表达,IQGAP1蛋白可以通过提高肺癌细胞的运动能力促进肺癌的侵袭转移。结论 IQGAP1与肺癌密切相关,可能在肺癌的侵袭和转移中起重要做用。

Objective To investigate the expression of Scaffold protein IQGAP1 in lung cancer,and the influence to cell invasion and metastasis.Methods The expression of IQGAPI mRNA was studied by RT-PCR,the expression of IQGAPl Protein was analyzed by immunohistochemistry and western blot,invasion and metastasis of IQGAP1was test by Transwell.Results The abnormal high expression of IQGAP1 in lung cancer tissues compared with non-cancerous tissues.The IQGAP1 expression was positive correlated with the survival of lung cancer.IQGAP1 promote lung cancer invasion and metastasis by improve cancer cell motility.Conclusion The IQGAP1 expression is closely related to lung cancer,may play important role in invasion and metastasis in human lung cancer.