差异蛋白质组学及其在植物盐胁迫响应研究中的应用

Differential proteomics and its application in plant salt stress

随着蛋白质组学技术的发展,差异蛋白质组学的研究已引起国内外学者的广泛关注。介绍了植物差异蛋白质组学的产生背景及在凝胶和非凝胶技术方面的最新技术方法,植物差异蛋白质组学近年来正从定性向精确定量的方向发展。综述了差异蛋白质组学在植物盐胁迫响应研究中的最新进展,指出了差异蛋白质组学在植物盐胁迫响应研究中依然存在着过多地依赖于双向电泳-质谱(2DE-MS)技术,研究对象不多,不能有效分离相关蛋白质,差异蛋白质丰度分析不精确等问题。最后,提出将差异蛋白质组学和基因组学技术相结合来研究植物盐胁迫响应,从分子水平上系统诠释植物耐盐机制是今后研究的方向。

With the development of the proteomics technology,differential proteomics has drawn more and more attentions of scholars both at home and abroad.Background and the latest development of gel technology and non-gel technology of the differential proteomics are highlighted in this paper.The recent research progresses on the application of differential proteomics in plant salt stress are reviewed.The previous studies have some deficiencies such as too much dependence on the 2DE-MS techniques,limited number of research objects,ineffective separation of related proteins,and inaccuracy in abundance analysis of differential proteomics.The prospects for research on plant salt stress with the combination of differential proteomics and genomics techniques to illuminate the salt-tolerant mechanism at the molecular level are put forward.[Ch,33 ref.]