口炎清颗粒指纹图谱研究

The Fingerprint of Kouyanqing Granule

采用HPLC法构建了口炎清颗粒的指纹图谱,用Dikma PLATISIL ODS色谱柱,以乙腈-φ=0.1%甲酸溶液为流动相,检测波长为254 nm。该指纹图谱有23个共有特征峰,确认了其中7个色谱峰的成分分别为绿原酸、咖啡酸、木犀草苷、哈巴俄苷、肉桂酸、甘草酸铵、甘草苷。构建的指纹图谱,操作简便,专属性强、重复性好,为该产品的质量监控提供了有效手段。

In this paper,the HPLC fingerprint of Kouyanqing Granule was established with Dikma PLATISIL ODS（250 mm×4.6 mm,5 μm） column,acetonitrile and 0.1%（φ） formic acid as mobile phase,and the detection wavelength was 254 nm.23 common peaks were selected as the fingerprint peaks.Seven of these common peaks were identified as chlorogenic acid,caffeic acid,luteolin-7-O-glucoside,harpagoside,cinnamic acid,ammonium glycyrrhetate and glycyrrhizic acid.The method established is selective,simple and reproducible,which can be used for the quality control of Kouyanqing Granule.