滇重楼不同生长发育期化学成分的变化规律

Research on Changing Regulation of Ingredients in Paris polyphylla var. yunnanensis at Different Growth

目的:建立滇重楼HPLC指纹图谱,考察滇重楼不同生长期主要化学成分的变化。方法:采用高效液相色谱法。色谱柱为Agela Venusil XBP-C18柱(4.6 mm×250 mm,5μm);流动相乙腈-水,梯度洗脱;流速1.0 mL·min-1;检测波长210 nm;柱温35℃。结果:滇重楼中所含成分的各色谱峰均得到了有效的分离,在指纹图谱中共有峰20个,并指认了4个色谱峰。滇重楼化学成分随生长期的不同而各异。结论:该方法为滇重楼质量控制以及实现规范化种植提供了参考依据。

Objective：To establish HPLC chromatographic fingerprint of Paris polyphylla var. yunnanensis and investigate variation of the main components in samples at various collecting seasons. Method： The main components were separated on a Agela Venusil XBP-C18 （4.6 mm × 250 mm,5 μm） column and detected at 210 nm by gradient elution at the flow rate of 1.0 mL. min - 1 using acetonitrile-water as the mobile phase. Column temperature was at 35 ℃. Result ： A satisfactory method for HPLC fingerprint determination of P. polyphylla var. yunnanensis was obtained. The established fingerprint showed that 20 peaks were common and 4 peaks were identified. The main components of P. polyphylla var. yunnanensis differed from collecting seasons. Conclusion ： The method can be used for quality control and provide a valuable reference for Good Agriculture Practice of P. polyphylla var. yunnanensis.