期刊文献+

HPLC-ELSD法测定夜关门中D-松醇的含量 被引量:3

Determination of D-pinitol in Lespedeza cuneata by HPLC-ELSD
原文传递
导出
摘要 目的:建立高效液相色谱-蒸发光散射检测器(HPLC-ELSD)测定夜关门中D-松醇含量的方法。方法:样品用乙醇-水(70∶30)提取1.0 h后过滤,滤渣再用63%的乙醇-水提取1 h过滤,合并2次滤液并浓缩,定容;采用Cosmosil Sugar-D柱进行分离,乙腈-水(70∶30)为流动相,流速为1 mL·min-1,ELSD漂移管温度55℃,载气为氮气。结果:D-松醇在0.377~1.844μg呈现良好的线性关系(r=0.999 5),精密度为0.22%,加标回收率为96.78%。结论:该方法操作简单、准确度和灵敏度高、适合于D-松醇的检测和含量测定。 Objective:An analytical method was developed for determining D-pinitol in Lespedeza cuneata by HPLC-ELSD. Method : Sample was extracted by ethanol-water solution (70:30 by volume) for 1.0 h, then filtered and the residue was extracted by ethanol-water solution (63:37 by volume) for another hour, filtered again and combined the filtrate then concentrated it,and diluted it into 100 mL. The separation was performed on a Cosmosil-sugar D column with the mobile phase consisting of water and aeetonitrile (20:80 by volume). And the flow rate was 1 mL . min-1, the temperature of drift tube was 55 ℃ with Nitrogen as developing solvent. The linear calibration curve was obtained in the range of 0. 377-1. 844 μg with a linear correlation coefficient(r) of 0. 999 5. The RSD is 0.22% , and the add mark recovery was 96.78%. This method is simple, high sensitivity and good precision,it is suitable for determation D-pinitol in Lespedeza cuneata.
出处 《中国实验方剂学杂志》 CAS 北大核心 2011年第5期80-82,共3页 Chinese Journal of Experimental Traditional Medical Formulae
基金 安徽省国际合作项目(2009GH027)
关键词 夜关门 D-松醇 高效液相色谱-蒸发光散射法 Lespedeza cuneata D-pinitol HPLC-ELSD
  • 相关文献

参考文献12

二级参考文献38

  • 1邓海根,曹雨震.高效液相色谱仪的通用型质量检测器──蒸发光散射检测器(ELSD)[J].药物分析杂志,1994,14(3):61-63. 被引量:56
  • 2曹雨震,周倩如.蒸发光散射检测器及其应用[J].现代商检科技,1995,5(6):46-51. 被引量:5
  • 3冯埃生,邹汉法,汪海林,李瑞江,王学政,张玉奎.影响高效液相色谱/挥发激光散射检测器检测性能基本因素的考察[J].药物分析杂志,1996,16(6):414-417. 被引量:102
  • 4-.蒸发光散射检测器提高HPLC测定甾体化合物的灵敏度和准确性[J].药物分析杂志,1995,15(5):61-61.
  • 5Smimoff N, Cumbes Q J. Hydroxyl radical scavenging activity of compatible solutes[ J]. Phytochemistry, 1959, 25:1057 -1060.
  • 6Nguyen A, Lamant A. Pinitol and myo-inositol accumulation in water-stressed seedlings of maritime pine[ J ]. Phytoehemistry, 1988, 27:3423 -3427.
  • 7Sun W Q, Li X, Ong B. Preferential accumulation of D-pinitol in Acrostichum aureum gametophytes in response to salt stress[ J]. Physiologia Plantarum, 1999, 105 : 51 - 57.
  • 8Guo C, Oosterhuis D M. Pinitol occurrence in soybean plants as affected by temperature and plant growth regulators[ J]. Journal of Experimental Botany, 1995, 46 : 249 - 253.
  • 9McManus M T, Bieleski R L, Caradus J R, et al. Pinitol accumulation in mature leaves of white clover in response to a water deficit[J]. Environmental and Experimental Botany, 2000, 43 : 1 1 - 18.
  • 10Murakeozy e P, Smirnoff N, Nagy Z, et al. Seasonal accumulation pattern of pinitol and other carbohydrates in Limonium gmelini subsp. Hungarica[ J]. Journal of Plant Physiology, 2002, 159 : 485 -490.

共引文献56

同被引文献28

  • 1李银花,刘仲华,黄建安,龚雨顺.高效液相色谱-蒸发光散射检测器测定茶叶中茶氨酸[J].茶叶科学,2005,25(3):225-228. 被引量:21
  • 2马小红,沈少林,韩凤梅,陈勇.大黄蒽醌类化合物电喷雾质谱研究[J].湖北大学学报(自然科学版),2006,28(4):403-406. 被引量:37
  • 3胡凤銮,王在震,朱仁山,等.现代英汉药物词汇[M].2版.北京:人民卫生出版社,2003:1220.
  • 4Akiko Higashiyama, Hla Hla Htay, Makoto Ozeki, et al. Effects of L-theanine on attention and reaction time response [J]. J Func Food,2010,3(3):171.
  • 5Kawada S, Kobayashi K, Ohtani M, et al. Cystine and theanine supplementation restores high-intensity resistance exercise-induced attenuation of natural killer cell activity in well-trained men [ J ]. J Strength Conditioning Res, 2010,24(3) :846.
  • 6湖南省衡阳地区防治慢性气管炎协作组;湖南省衡阳地区革委会卫生局.夜关门结晶ⅠI号治疗慢性气管炎的初步总结[J]新医药学杂志,1973(11):19-24.
  • 7方志先;赵晖;赵敬华.土家族药物志[M]北京:中国医药科技出版社,2007651-652.
  • 8南京中医药大学.中药大辞典:上册[M]上海:上海科学技术出版社,20092034-2035.
  • 9Gomes C I,Obendorf R L,Horbowicz M. Myoinositol,D-chiro-inositol,and D-pinitol synthesis,transport,and galactoside formation in soybean explants[J].Crop Science,2005,(04):1312.
  • 10Lee J,Lee C,J eong Y. D-pinitol regulates Th1/Th2 balance via suppressing Th2 immune response in ovalbumin-induced asthma[J].FEBS Letters,2007,(01):57-64.

引证文献3

二级引证文献11

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部