HPLC同时测定鼠掌老鹳草中5种活性成分的含量

Simultaneous Determination of Five Active Compounds in Geranium sibiricum by HPLC

目的:建立HPLC同时测定鼠掌老鹳草中没食子酸,原儿茶酸,槲皮素,山奈素,山奈素-3,7-O-α-二鼠李糖苷的含量测定方法。方法:采用RP-HPLC,Agilent TC-C18色谱柱(4.6 mm×250 mm,5μm),0.4%磷酸水溶液-甲醇为流动相梯度洗脱,流速0.8 mL·min-1,检测波长为切换波长,0～23 min,270 nm;23～55 min,370 nm,柱温30℃。结果:没食子酸,原儿茶酸,槲皮素,山奈素,山奈素-3,7-O-α-二鼠李糖苷的线性范围0.192～1.732 g·L-1(r=0.999 7),0.016～0.256 g·L-1(r=0.999 9),0.004～0.039 g·L-1(r=0.999 9),0.004～0.041 g·L-1(r=0.999 9),0.200 4～1.603 2 g·L-1(r=0.999 8),平均回收率(n=3)分别为99.93%(RSD 1.5%),98.27%(RSD 1.7%),100.07%(RSD 2.2%),99.92%(RSD 1.8%),99.63%(RSD1.0%)。结论:该含量测定的方法快速、准确、可靠、重复性好,可为药材的质量控制提供依据。

Objective：To determine 5 active compounds in Geranium sibiricum,namely gallic acid,protocat-echuin acid,quercetin,kaempferol-7-O-rhamnoside and kaemferitrin.Method：The HPLC method was applied and the separation was performed on an Agilent TC-C18 analytical column（4.6 mm × 250 mm,5 μm）.The mobile phase was methanol（A）-water containing 0.4% H3PO4（B） with gradient elution mode at the flow rate of 0.9 mL·min-1.The detection was set at 270 nm（0-23 min）,370 nm（23-55 min）,and the column temperature was 30 ℃.Result：The liner ranges of gallic acid,protocatechuin acid,quercetin,kaempferol-7-O-rhamnoside and kaemferitrin were 0.192-1.732 g·L-1（r = 0.999 7）,0.016-0.256 g·L-1（r = 0.999 9）,0.004-0.039 g·L-1（r = 0.999 9）,0.004-0.041 g·L-1（r = 0.999 9）,0.200 4-1.603 2 g·L-1（r = 0.999 8）.The average recoveries（n = 3） were 99.93%（RSD 1.5%）,97.27%（RSD 1.7%）,100.07%（RSD 2.2%）,99.92%（RSD 1.8%）,99.63%（RSD 1.0%）.Conclusion：The methord is simple and accurate with a good reproducibility and can be used as a quality control methord for G.sibiricum of different sources.