HPLC同时测定地黄中梓醇与毛蕊花糖苷的含量

HPLC Simultaneously Determination of Content About Catalpol and Verbascoside in Rehmannia

目的:研究地黄中梓醇与毛蕊花糖苷的含量测定方法,为其质量评价提供依据。方法:采用HPLC梯度洗脱法,在同一色谱条件下测定含量。色谱条件:AgilentSB-AQ色谱柱(4.6 mm×250 mm,5μm),流速1 mL·min-1,柱温25℃,检侧波长210 nm。结果:两种成分均能达到基线分离,线性良好。结论:经过系统的方法学考察,该方法操作简便、结果准确、重现性好,适用于地黄2010年版药典规定的这2种苷类成分的含量测定。

Objective：To study the determination method about the catalpol and verbascoside,provide basis for quality evaluation. Method：Gradient elution was applied in HPLC analysis. Agilent SB-AQ chromatographic col- umn （4.6 mm × 250 mm,5μm） , velocity 1 mL. min - l , column temperature was 25 ℃ , wavelength was 210 nm. Result： Two components can achieve good baseline separation, linear. Conclusion：After the systematic methodology, the method is simple, accurate,reproducible,applicable to the provisions by pharmacopoeia rehmannia glutinosa two content determination.