摘要
目的:采用蒸发光散射检测器(ELSD)测定百合中薯蓣皂苷的含量。方法:以AKZONOBEL Kromasil C18(4.6 mm×250 mm,5μm)为色谱柱,乙腈-0.2%甲酸(45∶55)为流动相,流速1.0 mL·min-1,漂移管的温度46℃,载气(N2)1.56 bar。结果:薯蓣皂苷在3.31～26.5μg有良好线性关系,平均回收率为99.2%,RSD 1.02%。结论:该方法简单,分离效果好,结果准确,可用于百合药材中薯蓣皂苷的含量测定。
Objective：To estahlish a method for the determination of the content of dioscin in Bulbus Liliiusing high-performance liquid chromatography with evaporative light scattering detector（HPLC-ELSD）.Method：HPLC-ELSD analysis was performed on Akzonobel Kromasil C18（4.6 mm × 250 mm,5 μm）with a mixture of acetonitrile（A） and 0.2% formic acid water（B）（45：55） as the mobile phase.Drift tube temperature was 46 ℃,pressure of nebulizer gas was 1.56 bar.Result：The resolution of dioscin was good.The linear calibration curves were obtained over the range of 3.31-26.5 μg.The average recovery of the method was 99.23% with RSD of 1.02%.Conclusion：This method is convenient,reliable and can be used reliably for analyzing dioscin in bulbus Lilii.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第5期110-112,共3页
Chinese Journal of Experimental Traditional Medical Formulae
