莪术油对鼻咽癌细胞X-连锁凋亡抑制蛋白mRNA表达的影响

Effect of Zedoary Oil on X-linked Inhibitor of Apoptosis Protein mRNA Expression in Human Nasopharyngeal Carcinoma Cell

目的:研究莪术油对鼻咽癌细胞X-连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis protein,XIAP)mRNA表达的影响及其作用机制。方法:体外培养鼻咽癌CNE-2细胞,MTT(噻唑蓝)法观察莪术油(400,300,200,100 mg·L-1)对CNE-2细胞的抑制作用,RT-PCR法检测莪术油对CNE-2细胞XIAPmRNA表达的影响。结果:MTT法结果显示,莪术油对CNE-2细胞的抑制率呈时间-剂量依赖性(P<0.01)。与对照组相比,莪术油139.41 mg·L-1组XIAPmRNA表达水平明显降低(P<0.01)。结论:莪术油能明显抑制鼻咽癌CNE-2细胞生长,其机制可能是通过抑制XIAP基因的表达。

Objective：To investigate the effect of zedoary oil on XIAPmRNA expression in nasopharyngeal carcinoma cell and its mechanism.Method：Using the MTT assay to display the sensitivity to the drug,the inhibition of zedoary oil（400,300,200,100 mg·L-1） to nasopharyngeal carcinoma cell（CNE-2）in vitro was observed.XIAP mRNA expression was tested by RT-PCR assay in CNE-2 cell.Result：The inhibition rate of zedoary oil to CNE-2 indicated both dose-dependent and time-dependent antiproliferative effect by MTT assay（P 〈 0.01）.Compared with control group,the expression level of the XIAPmRNA was decreased obviously in the experiment group（zedoary oil 139.41 mg·L-1）（P 〈 0.01）.Conclusion：The proliferation of CNE-2 cell was inhibited obviously by zedoary oil and the mechanism may be related to inbhibition of expression of XIAP.