高危型人类乳头瘤病毒-16、-18在喉鳞状细胞癌中的检测分析

Detection of high-risk human papillomavirus types -16 and -18 in laryngeal squamous cell carcinoma

目的探讨人类乳头瘤病毒（HPV）感染与喉鳞状细胞癌（LSCC）的关系。方法采用多对引物进行组织DNA聚合酶链反应（PCR）及原位杂交（ISH）技术对84例LSCC组织进行检测，从多角度、多方面印证HPV的感染。结果PCR检出HPV—L1阳性率为27-4％（23／84），而针对HPV-16型、HPV。18型特异的E6／E7引物行PCR扩增的结果显示，29例（34．5％）为HPV-16型，6例（7．1％）为HPV-18型，其中4例（4．8％）为HPV-16、HPV-18混合感染。LSCC中HPV-16和HPV-18的总阳性率为36．9％。用地高辛标记的HPV-16E6探针进行的ISH结果显示喉癌组织中HPV-16E6mRNA的检出率为30．9％（26／84）。结论高危型HPV-16的感染可能参与LSCC的致癌过程，但其具体机制尚待进一步研究．

Objective To investigate the relationship between human papilloma virus （HPV） infection and the development of laryngeal squamous cell carcinoma（LSCC）. Methods To elucidate the role of HPV in the development of LSCC, we employed polymerase chain rcaetion（PCR） based on four pairs of primers and in situ hybridization （ISH）to screen the HPV infection in 84 LSCC tissues. Resu/ts Using HPV L1 general primer amplification, HPV DNA was detected in 23 （27.4 %） of the 84 LSCC samples, However, when specific primers for HPV-16 or -18 were used to amplify E6 and E7 in all samples, 29 cases （34.5 %） were positive for HPV-16, while 6 cases （7.1%） were positive for HPV 18. Coinfeetion of HPV-16 and -18 were found in 4 eases （4.8 %）. Overall, HPV type 16 and 18 infections were present in 36.9 % of the LSCC samples. In addition, the positive rate of HPV16 E6 mRNA was 30.9 % （26/84） in I,SCC by ISH with digoxin-labeled sense probes of HPVI6 E6. Conclusion High-risk HPV-16 may be an etiologic factor in the development of laryngeal squamous cell carcinoma, while the complicated molecular mechanism of HPV16 induced tumorgenesis needs a turther study.