白介素-1β基因TaqⅠ多态性在严重全身感染中的研究

IL-1β Taq Ⅰ polymorphism in severe sepsis

目的：调查白介素-1β（IL－1β）基因TaqⅠ多态性对细菌脂多糖（LPS）诱导的外周血单个核细胞的IL-1β基因表达的影响，及其与外科手术后并发严重全身感染易感性、预后的相关性。方法：以甘油醛-3-磷酸脱氢酶（GAPDH）为内参，逆转录聚合酶链反应技术（RT－PCR）半定量分析LPS诱导的外周血单个核细胞的IL-1βmRNA的表达水平。采用PCR、限制性内切酶分析、琼脂糖凝胶电泳结合溴乙淀染色法测定IL-1β基因TaqⅠ多态性，Southern印还法证实聚合酶链反应产物的特异性。93例术后严重全身感染患者和112名种族一致的健康对照者被纳入本研究对象。结果：IL-1βTaqⅠ多态性基因型和等位基因频率分布在本后并发严重全身感染的患者与健康对照组之间、在存活和死亡的患者之间没有显著性差异（P＞0.05）。LPS诱导外周血单个核细胞IL-1βmRNA的表达水平在IL-1βTaqⅠ多态性的三种型之间没有显著性差异（P＞0．05）。结论：IL-1β基因TaqⅠ多态性与本后严重全身感染的易感性及预后不相关，对IL-1β基因的转录水平不产生影响。

Objective: To investigate the effect of IL-1β Taq I polymorphism on the exprcssionof lL-1β mRNA in periphcral mononuclear cells stimulatcd by LPS, to detcrminc whether the IL-1βTaq I polymorphism is associatcd with susccptibilith to and outcomc of sevcre sepsis inpostoperation. Metheds: Ninety-thrce patients with diagnosis of sevcrc sepsis and one-hundred-twelve ethnically matched healthy individuals were included in this study. The level of lL-15 mRNAwas measured by semi-quantitative RT-PCR, GAPDH as an intemal standard. IL-lβ Taq I poly-morphism was typed using PCR folowed by restriction analysis, agarose gcl electrophoresis andcthidium bromide staining. The specificity of thc amplified DNA fragments was certificated bysouthern blot. Results: No significant difference in the allele frequency and genotype distribution ofthe IL-1β Taq I polymorphism was observed between patients with severe sepsis and normal individ-uals (P > 0.O5). The level of lL-1β mRNA did not differ between IL-1β Taq I polymorphicgenotypes (P >0 .O5). Conclusions: IL-1β Taql poymorphism was nether associated with the in-cidence nor with the outcomc of severe sepsis in optoperation. IL-l5 Taq I polymorphism did notaffect the transcription of IL-1β gene