HPLC法测定益母草药材中盐酸水苏碱含量

Determ ination of stachydrine hydrochloride in leonurus japonicus houtt by H]PLC

目的：建立高效液相色谱法（HPLC），测定不同批次益母草药材中盐酸水苏碱的含量。方法：采用磺酸基团键合硅胶的阳离子交换剂为填充剂；以0．05mol／L磷酸二氢钾溶液-磷酸-三乙胺（1000：1．5：0．4）为流动相；检测波长为192nm，流速为1．0ml／min，柱温25~C。益母草药材经适当提取纯化。结果：盐酸水苏碱在进样量为2．0996～18．8964μg范围内具有良好的线性关系，r=0．9997；盐酸水苏碱的平均回收率为98．62％，RSD为1．41％（n=5）。结论：本方法专属性强，重现性好，可作为益母草中盐酸水苏碱的定量测定方法。

Objective： To establish a HPLC method for the separation and determination of stachydrine hydrochlofide in leonurus japonicus houtt from different districts. Method： ？The Analytical column was Waters Spherisorb SCX column （250× 4.6mm,5μm）.The mobile phase was composed of O.05mol/L KH2PO4-H3PO4-（C2H5）3N （1000：1.5：0.4）; UV detection was performed at 192 nm, the flow rate wes 1.0mL/min, column temperature was at 25℃ .The test solution was obtained by sample extracted with ethanol and passed through an actined charcoal-aluminium oxide column using ethanol as eluant. Results： The calibration curve was linear over the range between 2.0996 and 18.8964ug of stachydrine （r=0.9997）;the average recover was 98.62 % and RSD was 1.41% （n=5）. Conclusion： This method is selective and reproducible, and can be used to determine stachydrine hydrechloride in leonurus japonicus houtt.