摘要
Computer assisted movement tracking was used to characterize the motility of two marine microalgae, lsochrysis galbana and Tetraselmis chui, and to investigate the toxicity of Cu, Pb, and Cd on motile percentage, curvilinear velocity, average path velocity, straight line velocity, linearity, straightness, and wobble. Except for motile percentage, all other motility parameters differed significantly between L galbana and T. chui. Based on relative motile percentage data, the median effective concentration （ECs0） of Cu on the motility of L galbana and T. chui was 31.4 and 1.3 gmol/L, respectively, while for Pb it was 37.8 and 10.9 ~tmol/L and for Cd it was 12 1.6 and 37.8 ~tmol/L, respectively. Compared to L galbana, T. chui was more sensitive to all tested metals. The toxic effect of the heavy metals on motility exhibited the following decreasing order for both species： Cu 〉 Pb 〉 Cd. Our results indicate that L galbana and T. chui motility is sensitive to heavy metals and can be used as an indicator for toxicology bioassays.
Computer assisted movement tracking was used to characterize the motility of two marine microalgae, lsochrysis galbana and Tetraselmis chui, and to investigate the toxicity of Cu, Pb, and Cd on motile percentage, curvilinear velocity, average path velocity, straight line velocity, linearity, straightness, and wobble. Except for motile percentage, all other motility parameters differed significantly between L galbana and T. chui. Based on relative motile percentage data, the median effective concentration （ECs0） of Cu on the motility of L galbana and T. chui was 31.4 and 1.3 gmol/L, respectively, while for Pb it was 37.8 and 10.9 ~tmol/L and for Cd it was 12 1.6 and 37.8 ~tmol/L, respectively. Compared to L galbana, T. chui was more sensitive to all tested metals. The toxic effect of the heavy metals on motility exhibited the following decreasing order for both species： Cu 〉 Pb 〉 Cd. Our results indicate that L galbana and T. chui motility is sensitive to heavy metals and can be used as an indicator for toxicology bioassays.
基金
supported by the National Department Public Benefit Research Foundation(No. nyhyzx07-047)
the Research Project of Zhejiang Education Department(No. Y200909301)
and the Open Fund of Zhejiang Key Laboratory of Exploitation and Preservation of Costal Bioresources(No. J2010002)
