不同放置条件下放置时间对临床尿液样本检测结果的影响

The effect of different storage conditions on the results of clinical urine analysis

目的:探讨临床尿液样本不同放置条件下的放置时间对检测结果的影响,以提高对尿液标本分析前的质量控制。方法:随机选取 33 名该院门诊就医病人,收集其随机尿液样本,分别检测尿生化、尿常规、尿流式相关检测指标。采用配对 t 检验和配对秩和检验进行统计分析。结果:在室温放置条件下,尿钠(Na)在 4h 显著升高(P <0.05);尿肌酐(CREA)、尿酸(UA)、白细胞平均前项散射光强度(WBC-MFsc)在 6h 出现明显变化(P <0.05),其中 CREA、UA显著升高,WBC-MFsc 显著下降;尿总蛋白(TP)在8h 显著升高(P <0.05),其余各检测指标在室温放置 8h 无显著性变化(P >0.05)。在 4℃冰箱保存条件下,尿钾(K)在 6h显著降低(P <0.05),尿 UA 在 8h显著降低(P <0.05);其余各检测指标在 8h 无显著性变化(P >0.05)。结论:不同放置条件下的放置时间对某些尿液检测结果可产生影响,在实际工作中应规范尿液标本检测流程。

Objective：To explore the effect of different storage conditions on the results of clinical urine analysis,and to improve urine pre-analysis quality.Methods：Thirty-three patients were randomly recruited from Anzhen Hospital.The participants＇urine samples were analyzed with the urine biochemical tests,routine urine tests and urine flow determination respectively.Paired t-test and Wilcoxon rank sum test were used in the statistical analysis.Results：When urine samples were conserved at room temperature,the values of sodium after 4h,Creatinine and uric acid after 6h increased significantly（P〈0.05）,while Leukocytes average scattering intensity referred to in the preceding paragraph after 6h decreased significantly（P〈0.05）,and total protein after 6h increased significantly（P〈0.05）.However,other values remained unchanged after 8h.Under the storage condition in refrigerator at 4℃,the values of potassium and uric acid decreased significantly after 6h and 8h respectively（P〈0.05）.Other values had no significant change after 8h（P〉0.05）.Conclusions：Storage conditions（temperature and time） may influence the results of clinical urine analysis.Therefore,the flow process of the urine analysis should be standardized in the practical work.