大鼠颅脑创伤后星形细胞早期反应特点

The Early Reactive Feature of Astrocytes after Traumatic Brain Injury in Rats

目的研究大鼠颅脑创伤后星形细胞的早期反应。方法用气体冲击导致大鼠局部脑挫伤，采用HE染色和透射电镜观察损伤后脑组织的病理变化，用胶质纤维酸性蛋白免疫组化染色检测反应性星形细胞。结果颅脑创伤后星形胶质细胞肿胀为一早期现象，反应性星形胶质细胞中胶质纤维酸性蛋白染色增强，伤后6小时，反应性星形细胞主要局限于伤区周围脑组织和伤侧海马，伤后24小时，星形细胞反应波及全脑，海马组织中尤为明显。结论颅脑创伤后星形细胞反应可能与早期胶质细胞应激反应有关，反应性星形细胞在继发性脑损伤病理过程中可能具有重要作用。

Obective To investigate the early reactive feature of astrocytes after traumatic brain injury in rats. Methods By using a gas impact device, the focal contusion of the brain was produced in rats. The pathomorphological changes of the injured brain tissue were for with HE staining and electromicroscope and the reactive astrocytes were examined by the immunolocalization of glial fibrillary acidic protein(GFAP). Results Astrocytes swelling wa an early phenamenon. Reactive astrocytes were defined by the presence of increased immunoreactivity to anti-GFAP. Six haurs after trauma, the presence of reactive astrocytes was restricted to the impact site and the lateral hippocampus. Twenty- four hours after trauma, reactive astrocytes were identified in the entire hemisphere.Conclusion These results suggest that the response of astrocytes is correlated to glial stress and reactive astrocytes may play an im portant role in the secondary brain injury.[