抑癌基因p16在卵巢肿瘤中的表达及其意义

Expression and Clinical Significance of Tumor Suppressor Gene p16 in Ovarian Neoplasm

采用ＳＰ免疫组化染色法对９７例卵巢癌，３５例卵巢良性肿瘤及１６例正常卵巢ｐ１６蛋白表达进行测定，结果ｐ１６蛋白表达阳性率分别为３８．１４％，８２．８３％和８７．５０％，卵巢癌组明显低于正常对照及良性肿瘤（Ｐ＜０．０１）；不同组织类型卵巢癌之间ｐ１６蛋白阳性率无显著性差异（Ｐ＞０．０５）；但随着分化程度的降低，ｐ１６蛋白阳性率明显降低，高分化组（５８．０６％）与低分化组（１３．０４％）有非常显著性差异（Ｐ＜０．０１）；Ⅰ、Ⅱ期癌和Ⅲ、Ⅳ期癌阳性率分别为５２．２７％和２６．４２％（Ｐ＜０．０５）；ｐ１６蛋白阳性的卵巢癌患者累计生存率明显高于ｐ１６阴性者（Ｐ＜０．０１）。说明ｐ１６基因表达缺失与卵巢癌的发生、分化程度、进展及预后密切相关。

In order to study relationship between tumor suppressor gene p16 expression and ovarian cancer,p16 protein was analyzed by SP immunohistochemistry in 97 cases of ovarian cancer,35 cases of benign tumor and 16 cases of normal ovaries.The results showed that the positive rate of p16 protein were 38.14%,82.83% and 87.50% respectively,and it was significant lower in ovarian cancer than in other tumors and normal control( P <0.05).In ovarian cancer,the presence of p16 protein was not related to histological types( P >0.05),and p16 positive rate in well differentiated ovarian cancer(58.06%) was significantly higher than those in poorly differentiated one(13.04%)( P <0.05).The rate in stage Ⅰ～Ⅱ and Ⅲ～Ⅳ was 52.27% and 26.42% respectively( P <0.05),and survival rate significantly decreased in p16 negative group compared with that in p16 positive group( P <0.01).These results suggest that p16 is an important tumor suppressor gene,the mutation and deletion of p16 expression are well related with the occurence,differentiation,development and poor prognosis of ovavian cancer.