蒙古族肥胖人群与ADRB1 G1165C、GNB3 C825T基因多态性交互作用的联合分析

A Associativity analysis betweem gene ploymorphisms of ADRB1 G1165C,GNB3C825T and the Mongolia obesity population

目的针对内蒙古蒙古族人群的ADRB1基因G1165C位点、GNB3基因C825T位点进行多因素分析,探讨ADRB1基因G1165C位点、GNB3基因C825T位点与蒙古族人群肥胖的相关性。方法采用Sequenom系统检测183例蒙古族健康人和90例肥胖患者的ADRB1基因G1165C位点、GNB3基因C825T位点的分布特点。结果 (1)肥胖组和对照组的临床特征经比较2组在年龄、饮酒、高血压家族史方面存在统计学差异,P<0.05。(2)基因型及等位基因频率分布比较:两组各基因型频率及等位基因频率分布符合Hardy-Weinberg平衡,达到遗传平衡,具有群体代表性。2组在ADRB1基因G1165C位点、GNB3基因C825T位点基因型及等位基因频率比较差异无统计学意义(P>0.05)。(3)经Logistic逐步回归分析后结果为2组基因型在肥胖的发病过程中无交互作用(P>0.05)。结论 ADRB1基因G1165C位点、GNB3基因C825T位点可能不是蒙古族人群患肥胖症的易感基因。

Objective：To investigate the association between G1165C site of ADRB1 gene,C825Tsite of GNB3 gene and the Mongolia obesity patients by multiplicity on G1165C site of ADRB1 gene,C825Tsite of GNB3 gene in Mongolia obesity patients.Methods：Sequenom system was used to test ADRB1 G1165C,GNB3 C825T gene polymorphisms of 90 Mongolia obesity patients and 183 Mongolia healthy controls.Results：（1） The age drinking and hypertension family history have statistics difference between two groups（P0.05）.（2）We compared genetype and the distribution of allele frequencies between case and control groups.The distribution of allele frequencies in patients with obesity and controls were in Hardy–Weinberg equilibrium.Achieve genetic balance and group representation.There were not significant difference in ADRB1 G1165C,GNB3 C825T genetype and allelic frequencies between obesity cases and controls（P0.05）.（3）Gradual regressive analysis show two groups genetype have not interaction in obesity onset process（P0.05）.Conclusion：G1165C site of ADRB1 gene and C825Tsite of GNB3 gene may be not predisposing gene sites of Mongolia obesity patients.