胃、肠癌及癌旁组织MDR1基因表达的研究

Gene expression of multiple drug resistance 1 in tissue of carcinoma of stomach,rectum,and tissue beside carcinoma

目的：探讨胃、肠癌的原发耐药性及多药耐药基因（ｍ ｄｒ１）的肿瘤特异性。方法：以未接受肿瘤化疗的 ３１ 例胃癌和 ２４ 例结、直肠癌患者的癌及癌旁组织为研究对象，以逆转录 Ｐ Ｃ Ｒ 技术和 Ｄｏｔ ｂｌｏｔ 技术进行 ｍ ｄｒ１ 基因 ｍ Ｒ Ｎ Ａ 表达的研究。结果：①未接受化学治疗的胃癌患者的癌组织ｍ ｄｒ１ ｍ Ｒ Ｎ Ａ 呈阳性表达者占 ４８３９％ （１５／３１），肠癌为 ５４１７％ （１３／２４）。②癌组织ｍ ｄｒｌ基因ｍ Ｒ Ｎ Ａ 高表达，癌旁组织的表达强度显著低于癌组织。结论：检测癌手术标本ｍ ｄｒ１ 基因可发现原发性耐药，对术后制定化疗方案有重要的参考价值。

Objective:We studied the primary drug resistance in carcinoma of stomach,and rectum,and tumor specificity of multiple drug resistance (mdr).Methods:Expression of mdr1 in carcinoma of stomach (n.31),rectum (n.24) without chemotherapy and tissue beside the carcinomas was studied by RT PCR and Dot blot technique.Results:①Positive expression in carcinoma of stomach was 48.39% (15/31);that in carcinoma of rectum,54.17% (13/24).②The intensity of expression of mdr1 mRNA was stronger in carcinoma tissue than that in tissue beside carcinoma.Conclusion:Examinating mdr1 gene of carcinoma has important reference value in selection of chemotherapeutic plan postoperation.We also prove that expression of mdr1 gene has obvious tumor specificity,which may be helpful in clinical prediction on prognosis.