三氯化钐、三氯化镨对小鼠腹腔巨噬细胞的影响

Influence of SmCl 3 and PrCl 3 on peritoneal macrophage of mice

目的：探讨小剂量三氯化钐（ Ｓｍ Ｃｌ３），三氯化镨（ Ｐｒ Ｃｌ３）对小鼠腹腔巨噬细胞（ Ｐ Ｍ ）形态结构及功能的影响。方法：选用雄性昆明小鼠，分别腹腔注射给予 ００５ ｍ ｇ·ｋｇ－ １，０５ ｍ ｇ·ｋｇ－ １ Ｓｍ Ｃｌ３和 Ｐｒ Ｃｌ３，连续７ ｄ。采用定量酶细胞化学、凝集素亲合细胞化学、透射电镜、扫描电镜及吞噬实验观察 Ｐ Ｍ 的结构和功能变化。结果：两种剂量 Ｓｍ Ｃｌ３ 、 Ｐｒ Ｃｌ３ 均使 Ｐ Ｍ 的酸性磷酸酶（ Ａ Ｃ Ｐ）、α醋酸萘酚酯酶（ Ａ Ｎ Ａ Ｅ）含量明显增加；刀豆蛋白 Ａ 受体（ Ｃｏｎ Ａ Ｒ）数目增多，吞噬中性红能力增强。电镜下 Ｐ Ｍ 胞质内溶酶体增多，表面皱褶及微绒毛增多。结论：小剂量 Ｓｍ Ｃｌ３、 Ｐｒ Ｃｌ３ 对小鼠 Ｐ Ｍ 的形态结构和功能有明显的促进作用。

Objective:To find out the influences of low dosages of samarium chloride (SmCl 3) and praseodymium chloride (PrCl 3) on structure and function of peritoneal macriphage (PM) in mice.Methods:The male Kunming mice were selected as experimental animals.They were given low dosages (0.05 mg·kg -1 ,0.5 mg·kg -1 ) of SmCl 3 and PrCl 3 through i.p for 7 days.The structure and function of PM were observed with quantitative cytochemistry,lectinaffinitive cytochemistry,transmission and scanning electron microscope and phagocytic test. Results:The two dosages of SmCl 3 and PrCl 3 not only increased the contents of ACP and ANAE and the number of ConA receptors in mice PM,but also improved the phagocytosis of PM on neutral red.Under electron microscope,the lysosomes were increased on plasma of PM and folds and microvilli on surface of PM were raised.Conclusion:The low dosages of SmCl 3 and PrCl 3 on structure and function of PM in mice had stimulated effects.