喉癌和癌旁组织核仁组成区形态计量分析和P_(53)蛋白表达

AgNORs cytometry and P 53 protein expression in carcinoma and adjacent tissue to carcinoma of larynx

目的：讨论肿瘤的浸润方式及 Ａｇ Ｎ Ｏ Ｒｓ 染色和 Ｐ５３ 蛋白表达在临床中的应用。方法：应用 Ｐｌｏｔｏｎ 一步法 Ａｇ Ｎ Ｏ Ｒｓ 染色和 Ｐ５３ 蛋白免疫组化方法观察 １９ 例喉癌、癌旁和相对正常粘膜中 Ｎ Ｏ Ｒｓ 的颗粒计数（ Ｇ Ｃ）、颗粒面积（ Ｇ Ｓ）和 Ｐ５３蛋白表达。结果：喉癌的 Ｇ Ｃ 和 Ｇ Ｓ最高，癌旁次之，相对正常粘膜最低，癌旁的 Ｇ Ｃ 和 Ｇ Ｓ有 ５ 例在均值以上。 Ｐ５３蛋白在 １９ 例喉癌中有 １４ 例呈阳性，在癌旁组织中有 ７ 例呈阳性。结论：喉癌的发展是多灶性和跳跃性的。手术的安全缘应该用 Ａｇ Ｎ Ｏ Ｒｓ染色和 Ｐ５３蛋白免疫组化方法确定。

Objective:The paper is to study the infiltrative pattern of tumor by P 53 protein immunochemical staining and AgNORs staining.Methods:We tested granular counts (GC) and granular square (GS) cytometrically and P 53 protein expression in carcinoma (CA),adjacent tissue to carcinoma (ATC) and relative normal tissue (RNT).Results:GC anjd GS were highest in CA and Lowest in RNT.The five of 19 cases in ATC were positive in Ag NORs staining and the seven of that were positive in P 53 protein staining.Conclusion:The infiltrative pattern of larygeal carcinoma was polyfocal and jumpy.The safe edge must be determined by P 53 protein and AgNORs staining for the purpose of the operation.