Ⅳ型胶原纤维和P_(53)蛋白在喉癌和不典型增生上皮中的表达

Expression of collagen Ⅳ and P 53 protein in carcinoma and dysplasia of larynx

目的：探讨 Ｐ５３基因对Ⅳ型胶原纤维（ ＣｏⅣ）的影响及临床诊断意义。方法：用免疫组化方法观察 ＣｏⅣ和 Ｐ５３蛋白在１９ 例喉癌（ Ｃ Ａ）和３４ 例不典型增生（ Ｄ Ｓ）中的表达。结果： ＣｏⅣ表达呈线型， Ｐ５３ 蛋白表达呈核型，两者在 Ｃ Ａ 中呈负相关性。在 Ｄ Ｓ中 ＣｏⅣ表达率为 ４４％ ， Ｐ５３蛋白表达率为５８％ 。结论： ＣｏⅣ和 Ｐ５３蛋白在喉癌中均有表达，当 Ｐ５３蛋白超表达时， ＣｏⅣ的量明显减少； ＣｏⅣ免疫组化可以做为 Ｐ５３蛋白在 Ｄ Ｓ诊断中的辅助指标。

Objective:The paper is to disccuss the influence of p 53 gene to Collagen Ⅳ.Methods:We observed the expression of collagen Ⅳ and p 53 protein in 19 cases of carcinoma and 34 cases of dysplasia.Results:The pattern of p 53 antigenicity was nuclear type and that of collage Ⅳ was line type.The expression rate of collage Ⅳ was 40 4% and that of p 53 protein was 58 3% in dysplasia.Conclusion:There was the negative relationship between the expressions of collagen Ⅳ and p 53 protein in carcinoma;the amount of collagen Ⅳ decreased when p 53 protein was overexpressed;immunohistochemical staining of collagen Ⅳ could be an assitant marker in prematignant diagnosis.