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TLR2和PLGF在特发性胎儿生长受限中的表达及意义 被引量：5

Expressions and significances of TLR2 and PLGF in idiopathic fetal growth restriction
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摘要 目的：探讨特发性胎儿生长受限（IFGR）患者母血及脐血中胎盘生长因子（PLGF）及胎盘组织中Toll样受体2（TLR2）的表达及意义。方法：选取郑州大学第三附属医院2008年12月2009年10月剖宫产分娩的FGR孕妇28例作为实验组,同期因社会因素剖宫产分娩的正常足月孕妇30例作为对照组。采用双抗体夹心酶联免疫吸附法（ELISA）测定实验组与对照组母血、脐血PLGF浓度;免疫组化SP法检测两组胎盘组织中TLR2的表达（平均灰度值为检测指标）。结果：①IFGR组母血、脐血PLGF浓度与对照组比较,明显降低（P〈0.05）;②胎盘组织中均有TLR2蛋白不同程度的阳性表达;③TLR2在胎盘合体滋养细胞上的表达实验组高于正常对照组,差异有统计学意义（P〈0.05）;④IFGR组母血中PLGF与胎盘组织中TLR2呈负相关。结论：母血、脐血PLGF减少可能是引起FGR的原因之一。IFGR组胎盘组织中TLR2表达增加可能是IFGR发病的重要因素之一。在IFGR中TLR2可能通过对PLGF的表达下调,抑制胎盘胎儿的发育。 Objective：To explore the expressions and significances of placental growth factor（PLGF） in maternal blood and umbilical cord blood and Toll-like receptor 2（TLR2） in placental tissues in cases with idiopathic fetal growth restriction（IFGR）.Methods：28 pregnant women with IFGR who delivered in the hospital from December 2008 to October 2009 were selected as experimental group,30 normal full-term pregnant women who delivered in the hospital because of social factors at the same period were selected as control group;double antibody enzyme-linked immunosorbent assay（ELISA） was used to measure the concentrations of PLGF in maternal blood and umbilical cord blood;the expressions of TLR2 in placental tissues of the two groups were detected by immunohistochemical SP method.Results：The concentrations of PLGF in maternal blood and umbilical cord blood in IFGR group were significantly lower than those in control group（P〈0.05）.TLR2 protein expressed in placental tissues to a different extent.The expression level of TLR2 in syncytiotrophoblast cells of placenta was significantly higher than that in control group（P〈0.05）.There was a negative correlation between PLGF in maternal blood and TLR2 in placental tissues.Conclusion：The decrease of PLGF in maternal blood and umbilical cord blood may be a cause of IFGR;the increase of TLR2 expression in placental tissues of IFGR group may be an important factor of pathogenesis of IFGR;TLR2 inhibits the development of placenta and fetus by down-regulating PLGF in cases with IFGR.
作者 田燕妮 崔世红 程国梅 霍会蚕 张林东 刘萍 张婷
机构地区 郑州大学第三附属医院妇产科
出处 《中国妇幼保健》 CAS 北大核心 2011年第3期425-427,共3页 Maternal and Child Health Care of China
基金 河南省科技攻关计划项目〔2010A320021〕
关键词 特发性胎儿生长受限 胎盘 胎盘生长因子 TOLL样受体2 Idiopathic fetal growth restriction Placenta Placental growth factor Toll-like receptor 2
分类号 R714.43 [医药卫生—妇产科学]
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参考文献10

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二级参考文献14

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共引文献2

同被引文献52

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中国妇幼保健
2011年 第3期

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