胰岛素对局灶性脑缺血再灌注损伤的作用

The effect of insulin on reperfusion injury after focal ischemia

目的 观察胰岛素对脑缺血再灌注损伤的治疗作用。方法 制备易卒中型肾血管性高血压大鼠（ Ｒ Ｈ Ｒ Ｓ Ｐ） ，用线栓法复制大脑中动脉阻塞（ Ｍ Ｃ Ａ Ｏ） ，造成缺血６ ｈ 再灌注１８ ｈ ，术后立即及６ 小时后即时使用胰岛素，测定神经功能障碍评分及脑梗死体积的变化。结果 胰岛素可使神经功能障碍评分显著减低，梗死灶体积及其占全大脑体积比值，两半球体积差值显著减小。结论 胰岛素能减轻脑缺血再灌注损伤，早期用药效果更好。

Objective To study the effect of insulin treatment on reperfusion injury after ischemia.Methods The renovascular hypertension rats (RHRs) with MCAO as described by Zea Longa for 6 hours and reperfusion for 18 hours. The presence of neurological deficit and infarct volume was measured at 24 hours after MCAO following treatment with insulin immediately and 6 hours after reperfusion. Results The neurologic scores, total infarct volume, the percent of infarct volume in cerebral volume and the difference between both hemispheres were significantly lower in the groups treatment immediately after MCAO and reperfusion than the groups without treatment ( P<0.05 ). Conclusions Insulin may reduce reperfusion injury, and should be used as early as possible.