摘要
目的 探索新一代抗抑郁药万拉法新对大鼠下丘脑和海马内ｃｆｏｓ 和ｃｊｕｎ 蛋白表达的影响。方法 采用特异性抗体的原位免疫细胞化学方法，在强迫游泳大鼠抑郁模型上，观察万拉法新慢性给药（ 腹腔内注射每日１ 次，连续７ 次）对大鼠游泳不动时间和下丘脑及海马核团ｃｆｏｓ 和ｃｊｕｎ 表达的影响；用图像分析技术对大鼠下丘脑室旁核（ Ｐ Ｖ Ｎ） 、视上核（ Ｓ Ｏ Ｎ） 和海马齿状回（ Ｄ Ｇ） 内的ｆｏｓ 和ｊｕｎ 阳性细胞的相对切面面积比和平均目标灰度进行分析。结果 强迫游泳可使大鼠下丘脑和海马内多个核团的ｃｆｏｓ 和ｃｊｕｎ 蛋白表达水平增加，而万拉法新明显缩短了强迫游泳大鼠的不动时间。图像分析结果提示，万拉法新使强迫游泳大鼠下丘脑 Ｐ Ｖ Ｎ 和 Ｓ Ｏ Ｎ 及海马 Ｄ Ｇ 内ｆｏｓ 和ｊｕｎ 阳性细胞相对切面面积比明显降低（ Ｐ＜００５） ，而平均目标灰度显著增加（ Ｐ＜ ００１） 。结论 下丘脑 Ｐ Ｖ Ｎ、 Ｓ Ｏ Ｎ 和海马 Ｄ Ｇ 可能是介导抗抑郁药抑制大鼠绝望行为的重要中枢核团，ｆｏｓ 和ｊｕｎ 蛋白可能是抗抑郁药发挥受体后作用的传导物质。
Objective To explore the expression of c fos and c jun by venlafaxine in the hypothalamus and hippocampus of forced swimming rats.Methods The immunohistochemical method for localization of c fos and c jun protein was preformed to observe the distribution of c fos and c jun neurons within the hypothalamus and hipocampus of forced swimming rats with chronic treatment of venlafaxine (ip daliy for 7 days), and the computerized image technique was used to analyze the change in grey degree and relative sectional area of Fos and Jun positive neurons in the hypothalamic paraventricular nucleus(PVN), the supraoptic nucleus (SON) and the hippocampal dentate gyrus. Results The expression of c fos and c jun protein could be induced by forced swimming and venlafaxine could suppress significantly the accumulated immobility time of forced swimming. With computerized image analysis, the relative sectional area decreased ( P<0.05 ) and the grey degree increased significantly ( P<0.05 ) within the Fos and Jun positive neurons in PVN, SON and DG of forced swimming rats with treatment of venlafaxine. Conclusions PVN, SON and DG might be the important structural substrates in central nervous system mediating the antidepressants on behavior of despair in forced swimming rats, and the Fos and Jun protein might be the common substance of the signal transductioin process for post receptor action of antidepressant.
出处
《中国神经精神疾病杂志》
CAS
CSCD
北大核心
1999年第5期289-291,共3页
Chinese Journal of Nervous and Mental Diseases
关键词
抗抑郁药
万拉法新
强迫游泳
C-fos
C-JUN
Antidepressant Venlafaxine Forced swimming\
C fos C junF ⅠR expression by 83 6% while the liposome alone had no inhibitory effect on IGF ⅠR Inhibition (80 0%
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