TORCH-IgM抗体检测方法在产前检查中应用的对比研究

Contrastive research of TORCH-IgM antibodies detective methods

目的了解TORCH-IgM抗体检测方法的优缺点。方法于2010年5月至2010年6月期间来我科室进行产前检查的孕妇123例抽取血样,采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)、微粒子酶免疫法(microparticle enzyme immunoassay,MEIA)和胶体金免疫层析法(gold immunochromat ographic assay,GICA)分别检测TORCH-IgM抗体,并进行分析比较,以找出适合临床应用的筛查方法。结果 ELISA法、MEIA法和GICA法检测TORCH-IgM的阳性率见下表。结论 ELISA法、MEIA法和GICA法检测TORCH-IgM抗体阳性检出率之间的差别均无统计学意义(P>0.05)。

Objective： To realize merits and faults of TORCH-IgM antibodies detective methods.Methods： We sampled the blood of 123 pregnant women who took the routine gestational examine come to my department from 2010 May to 2010 June.We used Enzyme-linked immunoabsorbent assay （ELISA） ,microparticle enzyme immunoassay （MEIA） and gold immunochromat ographic assay （GICA） to detect IgM antibodies of TORCH,then analysis and comparison to find screen way for clinical application.Results： ELISA、MEIA and GICA detect positive rates of IgM antibodies of TORCH were as follow tabulation.Conclusion： It isnot statistic meaning to detect positive rates of IgM antibodies of TORCH with ELISA、MEIA and GICA.