人卵裂胚和囊胚的玻璃化冷冻及临床应用

Vitrified cryopreservation for human embryoes in cleavage or blastocyst stage and its preliminarily applications in clinic

目的探索人卵裂胚及囊胚玻璃化冷冻的有效性。方法来源于生殖中心IVF或ICSI助孕的共270个胚胎(卵裂胚167个,囊胚103个),用Cryoloop作为胚胎载体和相同的玻璃化方法进行冷冻、复苏和移植,观察其复苏存活率、临床妊娠率、植入率和流产率。结果卵裂胚和囊胚的复苏率、植入率和妊娠率分别为97.0%、33.1%、42.9%和96.1%、43.9%、52%,流产率分别为13.3%和15.4%。结论以Cryoloop为胚胎载体,使用相同的玻璃化冷冻方法对人类卵裂胚和囊胚均可获得同样理想的冷冻效果和临床结局。

Objective： To evaluate the efficacy of vitrified cryopreservation for human embryos in cleavage and blastocyst stages using cryoloop.Methods： 270 embryos （167 and 103 in cleavage and blastocyst stage respectively） ,from the hospital IVF center,were divided into two groups,as their stages,I.e.cleavage or blastocyst stage,for the later artifical shrinked must be done prior to be freezed,and then rates for thawing survival,implantation,clinic pregnancy and miscarrage were observed.Results： 97.0% ,33.1% ,42.9% ,13.3% and 96.1% ,43.9% ,52% ,15.4% ;in rates mentioned above,were observed in 70 and 75 transfer cycles,respectively for cleavage and blastocyst stage embryos.Conclusion： This protocol for vitrified cryopreservation,using cryoloop,can work well and suitable for cleavage stage embryos and blastocyst stage embryos as well.