RFLP区分中国小麦秆锈菌不同小种及致病型的应用研究

An Application of RFLP to Differentiating a New Virulent Type and Common Types of Puccinia graminis f. sp tritici in China

用3种DNA限制性内切酶（EcoRⅠ，PstⅠ和HindⅢ），分析了中国小麦秆锈菌新致病型21C3CTR（p11），普通致病型的小种21C3，34，34C2和致病型21C3CKH，34MKH，34MKG，34C2MKR的限制性酶谱。结果表明：供试秆锈菌的基因组DNA经过同一酶切后，不同小种或致病型都出现了相同限制酶谱带，而特定酶／小种（或致病型）组合又都有特异酶谱带。计算了小种间或致病型间的共有DNA片段的比率。初步说明了RFLP可以作为深入探讨小麦秆锈菌种内不同小种、致病型间以及新致病型DNA差异的手段。

The genomic DNA extracted from a new virulence 21C3CTR (p11) and common virulent types: races 21C3, 34 and 34C2 and pathotypes 21C3CKH, 34MKH, 34MKG and 34C2MKR of Puccinina graminis f. sp tritici in China was digested with the restricion endonucleases EcoRⅠ, Pst Ⅰand Hind Ⅲ. The fragments of the severed DNA were separated by electrophoresis. According to the electrophoretic patterns there appeared DNA restriction fragment length polymorphisms, and therefore the common DNA fragment ratios among or pathotypes were calculated, indicating that RFLP could be ed as a method of probing the DNA differences among races or pathotypes, or esp. new virulences.