马铃薯Y病毒的RT-PCR检测

Detection of Potato Virus Y by Reverse Transcription and Polymerase Chain Reaction

通过两种方法提取了烟草病叶中PVY病毒的RNA，然后针对PVY^N的CP基因序列，设计合成了一对特异性引物，运用反转录PCR技术对提取的PVY－RNA进行了体外扩增，结果得到与预期大小相一致的780bp片段，而对照未得到任何产物，从而建立了运用RT－PCR手段检测植物中PVY的又一有效途径，并运用此方法对东北三省部分地区马铃薯种薯中PVY的带毒情况进行了检测。

The potato virus Y (N strain) genomic RNA was obtained from PVY - infected tobacco leaves by using two methods, and a pair of primers were designed and synthesized based on the nucleotide sequence of the CP gene of potato virus Y (PVY) genome. By the reverse transcription and polymerase chain reaction (RT-PCR) a 780bp DNA fragment was amplified from PVY - RNA, while at the same time no fragment was obtained from the control. Those results revealed that the RT-PCR is a sensitive, specific and reliable method for the detection of PVY.