低氧对SD大鼠阴茎海绵体平滑肌纤维化的影响

Hypoxia induces fibrosis of corpus cavernosum smooth muscle in SD rats

目的:探讨低氧对SD大鼠阴茎海绵体平滑肌纤维化的影响。方法:体外培养阴茎海绵体平滑肌细胞,免疫组化鉴定细胞;常规氧浓度(21%O2浓度)分别培养12、24、48、72h作为对照,低氧(1%O2浓度)干预12、24、48、72h,RT-PCR分别测定各组TGF-β1、Ⅰ型胶原、Ⅲ型胶原的相对表达量。结果:体外培养的阴茎海绵体平滑肌细胞生长良好,抗平滑肌α-肌动蛋白单克隆抗体免疫组化染色阳性;RT-PCR结果提示TGF-β1、Ⅰ型胶原、Ⅲ型胶原的相对表达量在48h内与低氧时间成正相关,时间进一步延长不能增加其相对表达量。结论:在低氧环境下,SD大鼠阴茎海绵体平滑肌细胞的TGF-β1、Ⅰ型胶原、Ⅲ型胶原的相对表达量随时间的延长逐渐增加,48h达到最大值。低氧可导致SD大鼠阴茎海绵体平滑肌纤维化。

Objective：To explore the effect of hypoxia on the fibrosis of corpus cavernosum smooth muscle cells （CCSMC） in SD rats. Methods： CCSMCs were cultured in vitro, identified by immunohistochemistry, and then exposed to hypoxia at the concentration of 1%O2 for 12, 24, 48 and 72 hours. Those exposed to normal oxygen concentration for the corresponding lengths of time were used as the control. The relative expressions of TGF-β1, type Ⅰ collagen and type Ⅲ collagen were determined by RT-PCR. Results： The in vitro cultured CCSMCs grew well, and the anti-α-smooth muscle actin monoclonal antibodies were positive on immunohistochemical staining. The relative expression levels of TGF-β1, type Ⅰ collagen and type Ⅲ collagen were positively correlated with the time of hypoxia interference within 48 hours, and did not increase further with prolonged exposure. Conclusion： When exposed to hypoxia, the relative expressions of TGF-β1, type Ⅰ collagen and type Ⅲ collagen in the CCSMCs of SD rats increased with the length of time, and reached the peak at 48 hours. Hypoxia can cause fibrosis of CCSMCs in SD rats.