厚垣轮枝菌对多菌灵、乙草胺和威霸的反应

Response of Verticillium chlamydosporium to Fungicide Benomyl,and Two Herbides Acetochlor and Whip Supper

在培养基上研究了从大豆胞囊线虫的胞囊上分离到的厚垣轮枝菌（Verticilliumchlamydosporium）的两个菌株［菌株Ⅰ，从黑龙江省安达分离的（AV），菌株Ⅱ，从辽宁省沈阳分离的（VC）］常用杀菌剂多菌灵及除草剂已草胺和威霸的反应。试验结果表明：在低浓度的多菌灵（0．1，1μg·mL(-1)）处理下，菌株Ⅰ和菌株Ⅱ的菌丝生长几乎不受影响，或仅受到轻微地抑制。两菌株在其它较高浓度（10，50，100μg·mL(－1)）处理下都表现受到抑制．在观察的所有浓度的乙草胺处理对菌株Ⅰ均表现出不同程度地抑制，其中，在1，50μg·mL(－1)处理下受到抑制较轻；菌株Ⅱ仅在500μg．500μg·mL(－1)浓度处理下受到明显抑制，而在其它浓度（1，10，50，200μg·mL(－1)）抑制程度较轻。在用威霸处理试验中，菌株Ⅰ在浓度10μg·mL(－1)的处理下受到抑制较轻，在其它浓度（1，50，200，500μg·mL(－1)）的处理下均受到不同程度的抑制；菌株Ⅲ仅在200，500μg·mL(－1)的处理下受到完全抑制，在其它浓度处理下受到的抑制程度较轻。

Two strains of Verticillum chlamydosporium (Strain I from Heilonajiang Province, Strain Ⅱ from Liaoning Province) isolated from the cysts of soybean cyst nematode (SCN), Heterodera glycines were tested for the responsesof the hypha growth on PDA media in labotatory to the fugicide Benomyl and two herbicides Acetochlor and WhipSuper (fenoxaprop). The results showed that at low concentration of Benomyl (0.1, μg·mL-1), the hypha growth ofStrain Ｉ and Ⅲ was not effected or slightly inhibited. At other higher concentration (10, 50, 100μ·mL-1), the growthof both strains was strongly inhibited. At all the given concentration of Acetochior, the hypha growth of Strain Ｉ wasslightly inhibited, but slightly at 1, 50μg·mL-1, and the hypha growth of Strain Ⅲ was strongly inhibited only at500μg·mL-1 and inhibited slightly at other concentration (1, 10, 50, 200μg·mL-1). With the treatment of Whip Super, the hypha growth of Strain Ｉ was slightly inhibited at 10μg·mL-1, but inhibited at other concentration(1, 50, 200,500μg·mL-1). The growth of Strain Ⅱ was strongly inhibited only at 200, 500μg·mL-1, and inhibited slightly at otherconcentration.