抗氧化应激参与参附注射液预处理诱导的肾脏保护作用

Protective Effect of Shenfu Injection with Antioxidant System on Rats' Kidney after the Ischemia-reperfusion Injury

目的探讨抗氧化应激是否参与参附注射液预处理诱导的肾脏保护作用。方法健康成年雄性SD大鼠21只随机分为假手术对照组(Sham组)、肾脏缺血再灌注组(I/R组)和参附注射液组(SF组);SF组给予参附注射液10mL/kg腹腔注射,每日1次,连续给药7d。麻醉下行右肾切除后,用无损伤动脉夹钳夹左侧肾蒂60min,再灌注24h,制备肾缺血再灌注损伤动物模型。比较各组SD大鼠再灌注24h肾脏组织中超氧化物歧化酶(superonidedismu tase,SOD)水平、过氧化氢酶(catalese,CAT)和丙二醛(malonical aldehyed,MDA)含量。结果与Sham组相比,I/R和SF组肾脏组织SOD和CAT显著降低,而MDA明显升高(P<0.05);与I/R组比,参附注射液能明显增加SOD和CAT水平(P<0.05),降低MDA含量(P<0.05)。结论参附注射液预处理可增强缺血再灌注损伤肾脏组织抗氧化应激,其表现为增强SOD和CAT的活力,减少MDA的生成。

Objective To explore the protective effect of Shenfu injection combined with antioxidant system on rats’ kidney after ischemia-reperfusion injury.Methods Twenty-one male Sprague Dawley（SD） rats were randomly divided into 3 groups： sham operation group（sham group）,ischemia-reperfusion group（IR group）,and shenfu injection treated group（SF group）.The rats were anesthetized with valebarbitone.Bilateral kidneys were exposed through midline incision.The right kidney underwent the nephrectomy and left renal pedicels were occluded for 60 minutes with a traumatic mini-clamp and then unclamped for 24 hours.Animals in SF group received Shenfu injection（10 ml/kg） through intraperitoneal injection every day for 7 days.About 24 hours after reperfusion,superoxide dismutase（SOD）,CAT and malonical aldehyde（MDA） were measured.Results The levels of MDA were lower in SF group than those in IR group（P〈0.05）.The level of SOD and CAT in SF group increased more significantly than which did in IR group（P〈0.05）.Conclusion Our finding suggests that antioxidant system in SF group works more efficiently than IR group to overcome oxidative stress in renal ischemia-reperfusion injury