VEGF-C和FLT-4在结直肠腺癌中的表达

Expression of VEGF-C and FLT-4 in Colorectal Carcinoma and Correlation with Tumor Metastasis

目的:研究VEGF-C和FLT-4在结直肠腺癌、癌旁组织以及正常肠组织的表达,探讨其在结直肠腺癌淋巴转移中的关系。方法:收集结直肠腺癌癌灶、癌旁组织和正常肠组织标本,分别采用免疫组化、HE染色检测癌灶组和癌旁组、正常组的VEGF-C表达水平,癌旁组与正常组的淋巴管数,并分析二者与结直肠腺癌淋巴转移和临床病理特征之间的关系。结果:癌灶组和癌旁组、正常组的VEGF-C表达水平有显著性差异,癌旁组与正常组的FLT-4(阳性淋巴管数)表达水平具有显著性差异,其中VEGF-C免疫组化染色的阳性率与FLT-4阳性染色脉管数在TNM分期中的0～Ⅱ期和Ⅲ～Ⅳ期间有显著性差异。结论:联合检测VEGF-C和FLT-4在结直肠腺癌灶及癌旁组织中的表达,对结直肠腺癌淋巴转移有一定的预测价值,可以作为人结直肠腺癌淋巴转移的预测指标。

Objective To investigate the expression in colorectal adenocarcinoma of the relationship between the lymph node metastases,and to provide support for whether lymphatic cleaning and to postoperative chemotherapy should be done to patients. Methods The colorectal adenocarcinoma foci adjacent tissues and normal colorectal mucosa specimens were Collected and detected them respectively with PV9000 2-step immunohistochemical staining and HE staining：VEGF-C expression levels：the expression of VEGF-C in the primary foci of colorectal carcinoma, adjacent tissues and the normal group; the numbers of lymphatic in paraneoplastic group and normal group; and the relation between these two things and the lymphatic metastasis of colorectal adenocarcinoma and clinical pathological features was analyzed. Results The expressions of VEGF-C in colorectal adenocarcinoma foci, paraneoplastic group and the normal group are significantly different. The expressions of FLT-4 in paraneoplastic group and normal groups are strikingly different. The positive rate of the VEGF-C immunohistochemistry pigmentation and the number of FLT-4 masculine pigmentated vessels are quite different in the 0-II and III-IV periods in the TNM division. Conclusion To jointly detect the expression of VEGF-C and FLT-4 in the colorectal adenocarcinoma foci and paraneoplastic group of colorectal adenocarcinoma is quite valuable in the prediction of the lymphatic metastasis in human being’s colorectal adenocarcinoma. The joint detection can be used as prediction index to the lymphatic metastasis in human being’s colorectal adenocarcinoma and it is with great reference value especially to the treatment of the patients in clinical Dukes B period.