复黄生肌愈创油膏对大鼠糖尿病创面中Ⅰ型和Ⅲ型胶原mRNA表达的动态影响

Effects of Fuhuang Shengji Yuchuang(复黄生肌愈创)Ointment on Expressions of Type Ⅰ and Ⅲ Collagens mRNA in Granulation Wound Tissue in Rats with Diabetes

目的:动态观察复黄生肌愈创油膏对大鼠糖尿病创面新生肉芽组织中Ⅰ型和Ⅲ型胶原mRNA表达的动态影响。方法:36只雄性Wistar大鼠随机分为创面对照组、模型组、复黄膏组。采用荧光定量PCR技术,观察不同时段创面新生肉芽组织中Ⅰ型和Ⅲ型胶原mRNA表达的变化。结果:创面修复第11d,Ⅰ型胶原mRNA表达复黄膏组明显低于创面对照组(P<0.05),但和模型组比较无统计学差异;Ⅲ型胶原mRNA表达复黄膏组明显高于模型组(P<0.05),和创面对照组比较差异无统计学意义。结论:复黄生肌愈创油膏通过促进难愈性创面Ⅰ、Ⅲ型胶原增殖和平衡,从而起到促进创面愈合的作用。

Objective To observe the effects of Fuhuang Shengji Yuchuang （FHSJYC）（复黄生肌愈创）ointment, a compound traditional Chinese herbal medicine, on the expressions of type I and III collagens mRNA in wound granulation tissue in rats with diabetes. Methods Wistar rats were randomly divided into three groups： wound control group, normal saline （NS） group and FHSJYC group. Diabetes was induced by injection of 1.5% alloxan and oral gavage of 5% glucose, and skin wound was made in rats of the NS group and FHSJYC group. Skin wounds of the rats in the FHSJYC group were treated with FHSJYC ointment gauze dressing, while those in the NS group were treated with NS gauze dressing once daily. The rats were executed in turn on the third day and the eleventh day of the treatment, and the changes of type I and III collagen mRNA in the wound granulation tissue were observed by fluorescent real time quantitative polymerase chain reaction. Results Compared with the NS group, the wound closure index in the FHSJYC group was increased （P 0.05） After 3-day treatment , the expression of type I and III collagens mRNA showed no significant differences among the three groups. After 11-day treatment, the expression of type I collagen mRNAs in the FHSJYC group was lower than that in the wound control group （P 0.05）, similar with those in the NS group. The expression of type III collagen mRNA in the FHSJYC group was higher than that in the NS group （P 0.05）, similar with that in the wound control group. Conclusion FHSJYC ointment can affect the process of wound healing by promoting and regulating the expressions of type I and III collagens mRNA. These results show effects in accelerating wound healing and minimizing scar proliferation in uncurable wound.