表没食子儿茶素-3-没食子酸酯抑制TNF-α和IL-β在小鼠皮肤烫伤修复期间的表达

Epigallocatechin-3-gallate Inhibits Expression of Tumor Necrosis Factor-α and Interleukin-1β During Skin Scald Repair in Mice

肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白细胞介素-1β(interleukin-1β,IL-1β)在创伤修复中起着至关重要的作用.本研究利用小鼠皮肤深II度烫伤模型,采用逆转录聚合酶链反应(RT-PCR)和酶联免疫吸附试验(ELISA)检测烫伤部位组织Tnf-αmRNA和Il-1βmRNA的表达水平以及TNF-α和IL-1β的含量,探讨表没食子儿茶素-3-没食子酸酯(EGCG)对小鼠皮肤烫伤修复期间TNF-α和IL-1β表达的影响.结果显示,用0.2 mg/g EGCG膏剂涂敷烫伤皮肤,处理12 h可致组织Tnf-αmRNA表达水平和TNF-α含量下降,处理24 h可致组织Il-1βmRNA表达水平和IL-1β含量下降.上述结果提示,0.2 mg/g EGCG处理能抑制烫伤组织TNF-α和IL-1β的表达,减弱创伤组织的炎症反应,有助于创伤组织的修复.

The cytokines,tumor necrosis factor-α（TNF-α） and interleukin-1β（IL-1β）,play a vital role in wound healing.In the present study,levels of both Tnf-α mRNA and Il-1β mRNA were analyzed with reverse transcription polymerase chain reaction（RT-PCR）,and both TNF-α and IL-1β were detected with enzyme-linked immunosorbent assay（ELISA） in the mice skin model with full-thickness second-degree scald,to understand the effect of epigallocatechin-3-gallate（EGCG）,a main constituent of green tea polyphenols,on the expression of TNF-α and IL-1β during skin scald repair.The results showed that,after the scald injury skin were treated with 0.2 mg /g EGCG,the levels of both Tnf-α mRNA and TNF-α declines at post-scald for 12 hours,and both Il-1β mRNA and IL-1β decrease at post-scald for 24 hours.The data suggested that 0.2 mg /g EGCG inhibits the expression of Tnf-α and Il-1β gene,and attenuates inflammatory reaction at scald injury skin.Thus,EGCG contributes to the scald injury skin repair.