不同穴位电针对局灶性脑梗死大鼠梗死灶周围皮质GAP-43表达的影响

Effect of electroacupuncture of different acupoints on GAP-43 expression after focal cerebral infarction in rats

目的:比较督脉取穴法("人中"、"百会"穴)和背俞穴取穴法("肝俞"、"肾俞"穴)抗急性脑缺血损伤的效果。方法:成年健康雄性SD大鼠80只,采用线栓法造成右侧大脑中动脉阻断(Middle cerebral artery occlusion,MCAO)模型后,随机分为假手术组(n=5)、模型组(n=25)、电针人中+百会组(n=25)、电针肝俞+肾俞组(n=25)。选取24 h、3、7、14、21 d 5个时间点。通过Longa评分评价神经功能恢复情况,应用免疫组化染色、RT-PCR方法检测各组大鼠生长相关蛋白-43(Growth associate protein-43,GAP-43)及mRNA的表达情况。结果:假手术组无神经功能障碍,2电针组Longa评分明显优于模型组;GAP-43在假手术组不表达,缺血2 h再灌注24 h模型组出现GAP-43阳性表达细胞,并且呈先递增后减少的趋势。再灌注后7 d,模型组的GAP-43阳性细胞及其mRNA表达达到高峰,14 d时接近初始水平;而电针人中+百会组及肝俞+肾俞组的GAP-43表达仍维持较高水平(P<0.01),但2电针组之间差异无统计学意义(P>0.05)。结论:电针人中+百会组和肝俞+肾俞组可以促进MCAO大鼠脑损伤区轴突再生,2组针刺效果相近。

Objective：To investigate the effect of electroacupuncture（EA） of ＂Renzhong＋Baihui＂（RB）,＂Ganshu＋Shenshu＂（GS）resisting Acute cerebral ischemia（CI）.Methods：80 healthy adult male SD rats were randomly divided into sham-operated group（n=5）,model group（n=25）,RB group（n=25）,GS group（n=25）.MCAO was made by nylon monofilament suture,The rats in the EA group received EA treatment at the acupoints RB and GS after MCAO.The rats were randomly divided into different time points（24 h,3,7,14,21 d after reperfusion）.The neural functional recovery was evaluated by Longa＇s score.Imunohistochemistry and RT-PCR were used to detect the expression of GAP-43 in the ischemic cortex.Results：Sham-operated group had no neurological dysfunction,the Longa scores were better in both of the electroacupuncture（EA） groups than in model group.No positive GAP-43 cells were detected in sham-operated group.After 2 hour ischemia and 24 h reperfusion,positive GAP-43 cells were detected in the model group,and their expression showed increased first and then decreased.GAP-43 positive cells and mRNA expression in model group reached the peak on seventh day after MCAO,dropping to incipient level after 14 d.While the expression of GAP-43 in the two EA groups retained higher than model group（P0.01）.No significant differences were found between two EA groups（P0.05）.Inclusions：The electroacupuncture of ＂Renzhong＋Baihui＂ and＂Ganshu＋ Shenshu＂ may play an important role in the process of axonal regeneration after cerebral infarction,and the effect of acupuncture were similar in both groups.