血小板源生长因子A（PDGF—A）在发育性髋关节发育不良关节囊的分布和表达

Expression of platelet-derived growth factor-A in the hip capsule of children with developmental dysplasia of the hip

目的探求发育性髋关节发育不良患儿（devdopmentdysptasiaofhip，DDH）和对照组儿童血小板源生长因子A（PIX；FA）在关节囊中的免疫组化分布规律与水平差异，并比较PDGF-A在mRNA水平的表达差异，以探索髋关节松弛的原因。方法共收集了髋脱位患儿64例和对照组儿童14例，二组之间成功配对8组，进行配对研究，每组配对条件为性别相同、年龄相近，采用S-P法免疫组化技术和半定量RT-PCR技术检测关节囊中PDGF-A的分布规律与水平差异，以及在mRNA水平的差异。运用图像分析软件进行量化分析，并采用SPSS10.0软件进行统计学分析。结果可见分泌PDGF—A的成纤维细胞于关节囊内贴近关节侧的滑膜层呈强阳性表达，内部纤维层阳性表达细胞稀疏，强度明显减弱。8例患儿与对照组配对比较，纤维层中有阳性表现的成纤维细胞数占成纤维细胞总数的百分比（配对T检验P〈0．001）和局部染色的灰度值（配对丁检验P〈0．05）在二组间差异存在统计学意义，DDH组关节囊PDGF-AmRNA的表达较对照组减少（配对丁检验P〈0．01）。结论PDGF－A的分布减少和表达异常很可能与DDH患儿的髋关节松弛有关。

Objective To study the expression of platelet-derived growth factor-A （PDGF-A） in the hip capsule of children with developmental dysplasia of the hip （DDH）. Methods The clinical data of 64 patients with DDH and 14 control children were retrospectively analyzed. Among them, 8 pairs of DDH patients and their controls were chosen for this study. The tissues were collected during surgery. The PDGF-A in the hip capsule was detected using immunohistochemistry （SP method） staining. RT-PCR was used to study the mRNA expression of PDGF-A. Results High expression of PDGF-A was observed in the synovial layer with fibroblast regularly arranged parallel to the joint sur- face. PDGF-A expression was decreased in the fibrous layer of the hip capsule. The percentage of positive fibroblast and gray-scale density was significantly different in the fibrous layer of the DDH patients compared to controls （P〈0. 05）. The mRNA expression of PDGF-A was also decreased in the DDH patients compared to the controls （P〈0. 01 ）. Conclusions The decreased expression of PDGF- A in the hip capsule may involve in the development of DDH.