人肝癌肺和淋巴结靶向转移细胞及裸鼠移植模型的建立

The establishment of a systematic site-specific metastasis model of human hepatocellular carcinoma in nude mouse

目的克隆具有肺和淋巴结转移亲嗜性的人肝癌细胞并建立相应的裸鼠移植模型。方法将荧光人肝癌细胞HCCLM3－R接种于4周龄的裸鼠肝脏，6周后在荧光解剖下观察并获取肺及腹腔淋巴结转移灶组织，经体外克隆培养，所获细胞分别标记为HCCLM3R—LM1和HCCLM3R—LnMl。接种上述两种细胞于4周龄裸鼠肝脏，观察各自肺和腹腔淋巴结转移灶荧光数量，并与肺组织连续切片中的转移灶数目进行比较。计量资料采用Wilcoxon秩和检验和Kmskal—Wallis秩和检验进行统计学分析。结果HCCLM3-R—LM1、HCCLM3-R和HCCLM3-R—LnMl细胞接种于裸鼠肝脏后第6周，在肺和腹腔淋巴结中均能发现肿瘤转移。3株细胞接种后，裸鼠肺和腹腔淋巴结转移灶荧光面积分别为8687．00±1844．63和2570．00±318．20（P〈0．001），6457．67±832．62和10994．33±2212．31（P〈0．001），2968．67±2571．00和24416．00±7186．13（P〈0．001），每只裸鼠光镜下肺转移灶中位数分别为755、430、310个（P〈0．001），与荧光定量结果相吻合。结论成功建成人肝癌肺和淋巴结亲嗜性转移细胞和裸鼠移植模型，其中HCCLM3R—LM1细胞具有明显的肺转移特性，而HCCLM3－R—LnM1细胞具有明显的淋巴结转移特性，为肝癌器官靶向转移研究提供了理想的体内外模型。

Objective To establish a systematic site-specific metastatsis model of human hepatocellular carcinoma （HCC） in nude mouse. Methods HCCLM3-R cells were seeded into mice liver to establish xenograft mouse models. With the help of RFP, metastasis foci in lungs and lymph nodes in mice were detected using fluorescent stereomicroscopy and were removed. Cells derived from the metastasis foci were named HCCLM3-R-LM1 and HCCLM3-R-LnM1 respectively. HCCLM3-R-LM1 and HCCLM3-R-LnM1 cells were seeded into mice livers to analyze the lung and lymph node metastasis. Lungs of all tested mice were collected, examined by pathological evaluation and counted lung metastasis. Results Both lung and lymph node metastasis were found in HCCLM3-R-LM1, HCCLM3-R and HCCLM3-R-LnM1 ceils and a significant difference was found between the lung and the lymph node metastasis levels in the three cells. The fluorescent areas （pixels） of lung and lymph node metastasis were 8687.00 ± 1844.63 versus 2570.00 ± 318.20 （P = 0.0031） in HCCLM3-R-LMI cells, 6457.67 ± 832.62 versus 10 994.33 ± 2 212.31 （P = 0.0036） in HCCLM3-R cells, and 2968.67 ± 2571.00 versus 24 416.00 ± 7 186.13 （P = 0.0094） in HCCLM3- R-LnM1 cells, respectively. The middle numbers of microscopic lung metastatic foci were 775,430 and 310 in HCCLM3-R-LMI, HCCLM3-R and HCCLM3-R-LnM1 cells （P 〈 0.001）, respectively, consist with the results quantified by RFP. Conclusion We established the systematic site-specific metastasis models which demonstrates lung- and lymph node-specific metastasis potential in nude mice and can be used as a model for researches on site-specific metastasis of HCC.