辣木叶或根的甲醇提取物对弗氏佐剂致关节炎大鼠模型的止痛作用(英文)

Analgesic effects of methanolic extracts of the leaf or root of Moringa oleifera on complete Freund's adjuvant-induced arthritis in rats

目的:辣木(Moringaoleifera)是非洲民间医学中一种常用的药用植物,近期已有临床研究揭示了其抗炎功效。本实验研究辣木叶及根的甲醇提取物对弗氏佐剂致关节炎模型大鼠的止痛作用。方法:使用健康雄性成年Wistar大鼠(体质量200~220g),皮下注射弗氏佐剂建立实验性关节炎模型。造模当天及造模后第3天和第6天,治疗组大鼠分别腹腔内注射辣木根或叶的甲醇提取物(200、300或400mg/kg),使用消炎痛(5mg/kg)作为阳性对照药。每次用药后评估各组大鼠的温度痛觉过敏程度及机械性触诱发痛程度。实验用同样方法检测了辣木根和叶的甲醇提取物混合物(200mg/kg)的止痛作用。结果:辣木根或叶的甲醇提取物(300或400mg/kg)的止痛作用与消炎痛(5mg/kg)类似,与对照组比较,造模后第3天或第6天用药都能够显著降低弗氏佐剂致关节炎大鼠的温度痛觉过敏程度及机械性触诱发痛程度(P<0.01或P<0.05)。与对照组、辣木根提取物组及辣木叶提取物组比较,造模后第3天或第6天用药,辣木根和叶的甲醇提取物混合物(200mg/kg)能够显著降低弗氏佐剂致关节炎大鼠的温度痛觉过敏程度(P<0.01)。结论:辣木根或叶的甲醇提取物能够有效降低弗氏佐剂致关节炎大鼠的温度痛觉过敏程度。辣木根和叶的甲醇提取物混合物与根或叶单独的提取物的作用比较显示出辣木根与叶在止痛效果上的协同作用。

Objective： Moringa oleifera （family Moringaceae） has been widely used in African folk medicine, and researchers have recently revealed its anti-inflammatory effects in human. This study aimed to evaluate the analgesic properties of methanolic extracts of M. oleifera in complete Freund＇s adjuvant （CFA）-induced arthritis in rats. Methods： Adult male Wistar rats, weighing 200 to 220 g, were used in this study. Adjuvant arthritis was induced on day 0 by a single subcutaneous injection of CFA. The prepared extracts from both the root and leaf （200, 300 and 400 mg/kg） of M. oleifera were administered intraperitonealy to rats in the treatment groups O, 3 and 6 d after CFA injection and indomethacin （5 mg/kg） was used as a positive control drug. Thermal hyperalgesia and mechanical allodynia were evaluated for the analgesic effect O, 3 and 6 d after CFA injection. Combined methanolic root and leaf extracts of M. oleifera （200 mg/kg） were also tested for the analgesic effect. Results： The potency of the root or leaf extracts of M, oleifera （300 and ~00 mg/kg） was similar to that of indomethacin, resulted in significant reductions in both thermal hyperalgesia and mechanical allodynia in rats with CFA-induced arthritis compared with the control group after 3 and 6 d, respectively （P^O. 01 or P^O. 05）. Combined root and leaf extracts （200 mg/kg） of M. oleifera resulted in a significant reduction in thermal hyperalgesia compared with the control group after 3 and 6 d, respectively （P^0.01）. Prophylactic injections of combined root and leaf extracts of M. oleifera （200 mg/kg） resulted in a significant reduction in thermal hyperalgesia compared with the control group, the root extracts group, and the leaf extracts group after 3 and 6 d, respectively （P^O.01）. Conclusion： The methanolic extracts of the root or leaf of M. oleifera are effective in the reduc- tion of pain induced by CFA in rats. A comparison of single and combination therapies of root and leaf extracts also showed a synergistic effect on pain reduction.