磁性纳米纤维复合材料原位诱导体内成骨的研究(英文)

Enhancement of a magnetic nanofibrous composite scaffold for bone regeneration

目的:研究一种新型顺磁性的纳米纤维复合支架γ-Fe2O3/nHAP/PDLLA在弱磁场下体内诱导新骨形成的功效。方法:纳米纤维复合材料支架通过电纺丝方法制成,支架内部的微观结构用扫描电镜(SEM)进行表征。将支架植入兔横突根部骨缺损处并在12周后处死动物,应用组织学方法研究支架在动物体内原位诱导新骨形成和胶原蛋白沉积的情况。结果:与对照的nHAP/PDLLA纳米纤维支架相比,磁性纳米纤维复合支架上有更多的Ⅰ型胶原沉积,新骨的生成量也明显增加。结论:磁性纳米纤维复合支架能够促进骨缺损部位的新骨生成,在引导骨组织再生与修复方面具有应用潜能。

Objective：To investigate the function of inducing bone regeneration of a novel paramagnetic nanofibrous composite scaffold of γ-Fe2O3/nHAP/PDLLAin vivounder a weak applied magnetic field.Methods： The scaffold was fabricated with the composite by electrospinning technique.The microstructure of the scaffold was characterized by scanning electron microscopy.The scaffold was implanted in defects at the root segment of the lumbar transverse process on a rabbit model.Bone tissue samples were collected after 12 weeks of implant surgery.New bone formation in the defects was assessed using histological analysis in reference to a control nanofibrous composite of nHAP/PDLLA.Deposition of typeⅠ collagen fibers were examined by Sirius red staining.Results： There was newbone formation observed in the scaffold.TypeⅠ collagen was deposited abundantly on the scaffold.Together all,the bone regeneration was enhanced obviously in comparison with that induced by control scaffold of nHAP/PDLLA.Conclusion： The scaffold of γ-FeO/nHAP/PDLLAenhanced osteogenesis under a weak static magnetic field,and exhibited promising potential for use in bone repair.